不同果胶酶处理对户太八号葡萄酒品质的影响

Effect of different pectinase treatments on the quality of Hutai No.8 wine

为探究不同果胶酶种类和添加量对户太八号葡萄酒整体品质的影响,以户太八号为原料酿造干红葡萄酒,研究3种商业果胶酶(HC、EX-V、RF)在不同添加量(20 mg/L、40 mg/L、60 mg/L和80 mg/L)下对户太八号葡萄出汁率、葡萄酒澄清度、理化指标、甲醇含量、颜色和挥发性香气化合物的影响。结果表明,当果胶酶EX-V添加量为20 mg/L时,葡萄酒整体品质较好,葡萄酒的pH值、酒精度、挥发酸等指标无显著性影响(P>0.05),且甲醇含量满足GB/T 15037—2006《葡萄酒》标准要求,同时显著提高了葡萄的出汁率、葡萄酒的澄清度(P<0.05);由于总花色苷含量的提高,显著改善了葡萄酒颜色,具体表现在显著提高了色彩饱和度(C^(*)_(ab)值)、红色调(a^(*)值)(P<0.05),显著降低了黄色调(b^(*)值)(P<0.05);挥发性香气化合物含量显著高于其他处理组(P<0.05)。基于重要挥发性香气物质(OAV>0.1)可以区分不同果胶酶处理葡萄酒样。研究表明,添加20 mg/L果胶酶EX-V能显著提高户太八号葡萄酒的品质。

To investigate the impact of different types and additions of pectinase on the quality of Hutai No.8 wine,the dry red wine was produced using Hutai No.8 grapes as raw materials,and the effects of three commercial pectinases(HC,EX-V,RF)at different addition levels(20 mg/L,40 mg/L,60 mg/L and 80 mg/L)on the juice yield of Hutai No.8 grapes,and the clarity,physicochemical indexes,methanol content,color and volatile aroma compounds of Hutai No.8 wine were studied.The results revealed that the wine quality was excellent when the pectinase EX-V addition was 20 mg/L,the effects on the pH,alcohol content and volatile acid of wine were not significant(P>0.05),and the methanol content met the requirements of GB/T 15037—2006"Wine"standard.At the same time,the yield of grape juice and the clarity of wine were significantly increased(P<0.05).Moreover,an increase in total anthocyanin content led to a remarkable improvement in wine color,which showed that the color saturation(C^(*)_(ab) value),red hue(a^(*)value)were significantly increased(P<0.05),and the yellow hue(b^(*)value)was significantly decreased(P<0.05).Additionally,the content of volatile aroma compounds was significantly higher than that in other treatment groups(P<0.05).The wine samples with different pectinase treatments could be distinguished based on significant volatile aroma compounds(OAV>0.1).The results indicated that the addition of 20 mg/L pectinase EX-V could significantly enhance the quality of Hutai No.8 wine.