^(60)Co-NTG复合诱变选育丁烯基多杀菌素高产菌株及其杀虫活性

Breeding of Butenyl-spinosyns High Yielding Strain by^(60)Co-NTG Compound Mutation and Its Insecticidal Activity

为获得稳定高产的丁烯基多杀菌素生产菌株并明确其对农业害虫的杀灭效果,以前期研究获得的须糖多孢菌Saccharopolyspora pogona ASAGF19为初始菌株,通过^(60)Co-γ射线和亚硝基胍复合诱变得到1株遗传性状稳定的高产突变株ASAGF30A11,较初始菌株产量提高了61.26%,3次传代产量变化幅度小于10.00%。对高产菌株发酵液进行分离纯化获得了丁烯基多杀菌素粗品,配制成1.5 g/L浓度的母液进行稀释,对蓟马、蚜虫以及小菜蛾进行室内毒力测定,得出其对3种供试害虫的LC_(50)分别为1.92 mg/L、1.23 mg/L和0.27 mg/L。经过比较,丁烯基多杀菌素对蓟马的杀灭效果优于多杀菌素(LC_(50)为0.57 mg/L)和乙基多杀菌素(LC_(50)为0.64 mg/L);对蚜虫的杀灭效果优于多杀菌素(LC_(50)为72.60 mg/L),低于阿维菌素(LC_(50)为0.14 mg/L);对小菜蛾的杀灭效果优于多杀菌素(LC_(50)为2.87 mg/L),低于乙基多杀菌素(LC_(50)为0.05 mg/L)。本研究首次探索了^(60)Co-γ射线和亚硝基胍复合诱变对须糖多孢菌的影响,并获得了丁烯基多杀菌素对3种农业害虫的防效,为新型生物杀虫剂对农业害虫的防治提供了一些理论依据。

In order to obtain stable and high-yield butenyl-spinosyn-producing strains and determine its lethal efficiency against agricultural pests,a high-yield mutant strain ASAGF30A11 with stable genetic traits was obtained by compound mutagenesis of a parent strain Saccharopolyspora pogona ASAGF19 screened from the previous study using^(60)Co-γ-ray and nitrosoguanidine.The new strain increased yield by 61.26%compared with the parent strain,and the yield variation of 3 generations was less than 10%.The crude butenyl-spinosyn was purified from the fermentation broth of the high-yield strain,and then diluted into the mother liquor with a concentration of 1.5 g/L.The insecticidal activity against Thrips palmi Karny,aphids and Plutella xylostella was investigated and the LC_(50)was determined to be 1.92 mg/L,1.23 mg/L and 0.27 mg/L,respectively.By comparison,butenyl-spinosyn was of a higher lethal efficiency against T.palmi than spinosyn(LC_(50)0.58 mg/L)andspinetoram(LC_(50)0.64 mg/L),a higher lethal efficiency against aphids than spinosyn(LC_(50)72.60 mg/L)but equalefficiency with avermectin(LC_(50)0.14 mg/L),and a higher lethal efficiency against P.xylostella than spinosyn(LC_(50)2.9 mg/L)but lower efficiency than spinetoram(LC_(50)0.05 mg/L).This study explored the effect ofcompound mutagenesis using^(60)Co-γ-ray and nitrosoguanidine on S.pogona for the first time and measured thelaboratory lethal efficiency of butenyl-spinosyn against three agricultural pests,which serves as part of thetheoretical basis for the control of agricultural pests with new biological insecticides.