四磨汤对肝脾气滞型功能性消化不良大鼠胃肠动力的影响

Effects of Simo Decoction on the Gastrointestinal Dynamics of Rats with Functional Dyspepsia of Liver and Spleen Stagnation Syndrome

目的观察四磨汤对肝脾气滞型功能性消化不良(FD)大鼠血管活性肠肽(VIP)及其受体2(VIPR2)表达的影响,探讨其调节胃肠动力的作用机制。方法60只SD大鼠随机分为空白组、模型组、莫沙必利组(0.305mg/kg)和四磨汤高、中、低剂量组(5.62、2.81、1.40g/kg),采用多因素造模法制备肝脾气滞型FD大鼠模型,各给药组分别予相应药物灌胃14d。观察大鼠一般情况,测定体质量、胃排空率和小肠推进率,HE染色观察胃窦和十二指肠组织形态,免疫组化染色检测十二指肠组织VIP、VIPR2阳性表达,Westernblot、RT-PCR分别检测十二指肠组织VIP、VIPR2蛋白和mRNA表达。结果与空白组比较,模型组大鼠一般状况较差,体质量、胃排空率和小肠推进率均显著降低(P<0.01),十二指肠组织VIP、VIPR2蛋白和mRNA表达均显著升高(P<0.05,P<0.01);与模型组比较,四磨汤高、中剂量组和莫沙必利组大鼠体质量、胃排空率和小肠推进率均显著升高(P<0.05,P<0.01),十二指肠组织VIP、VIPR2蛋白和mRNA表达均显著降低(P<0.05,P<0.01)。HE染色显示,胃窦和十二指肠组织形态未发生明显改变。结论四磨汤可能通过抑制肝脾气滞型FD大鼠十二指肠组织VIP、VIPR2表达促进胃肠动力,从而改善FD。

Objective To observe the effects of Simo Decoction on the expressions of vasoactive intestinal peptide(VIP)and its receptor 2(VIPR2)in rats with functional dyspepsia(FD)of liver and spleen stagnation syndrome;To investigate its mechanism of action in regulating gastrointestinal motility.Methods Totally 60 SD rats were randomly divided into blank group,model group,mosapride group(0.305 mg/kg)and Simo Decoction high-,medium-and low-dosage groups(5.62,2.81,1.40 g/kg).The liver and spleen qi stagnation FD rat model was prepared by multifactorial modeling method,and the corresponding drugs were given by gavage for 14 days.The general conditions of rats in each group were observed,and body mass,gastric emptying rate and small intestine propulsion rate were measured;the histological structure of gastric antrum tissue and duodenal tissue were observed by HE staining;the positive expressions of VIP and VIPR2 in duodenal tissue were observed by immunohistochemistry;the protein and mRNA expressions of VIP and VIPR2 in duodenal tissue were detected by Western blot and RT-PCR,respectively.Results Compared with the blank group,the general condition of the rats in model group was poorer,body mass,gastric emptying rate and small intestine propulsion rate were significantly lower(P<0.01),and the protein and mRNA expressions of VIP and VIPR2 in duodenal tissue significantly increased(P<0.05,P<0.01).Compared with the model group,the body mass,gastric emptying rate and small intestine propulsion rate in Simo Decoction high-and medium-dosage groups and the mosapride group significantly increased(P<0.05,P<0.01),and the protein and mRNA expressions of VIP and VIPR2 in duodenal tissue significantly decreased(P<0.05,P<0.01).HE staining showed no significant changes in the morphology of gastric antrum and duodenum tissues.Conclusion Simo Decoction may promote gastrointestinal motility by inhibiting the expressions of VIP and VIPR2 in duodenal tissue of FD rats with liver and spleen qi stagnation,thereby improving FD.