不同蔬菜种植对虾菜共生系统中微生物群落结构的影响

Effects of Different Vegetable Cultivation on the Microbial Community Structure in Shrimp-vegetable Symbiotic System

[目的]研究蕹菜、甘薯、枸杞对虾菜共生系统中微生物群落结构的影响。[方法]通过高通量测序技术分析种植不同蔬菜的虾菜共生系统中微生物群落组成,并检测该系统中的硝酸盐、磷酸盐指标。[结果]不同虾菜共生系统中水体微生物共同的OTU数目为450种,其中,枸杞组(LM)的OTU数目最多,为852种,且Shannon-Wiener指数和Simpson指数最高;蕹菜组(IF)对硝酸盐、磷酸盐去除率分别达65.70%和67.57%,且与其他组群落结构差异显著(P<0.05)。在门水平上,不同系统中水体的优势菌群为变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、Patescibacteria;在属水平上,不同系统中的优势菌群为红球菌属(Rhodococcus)、不动杆菌属(Acinetobacter)、谷氨酸杆菌属(Glutamicibacter)。[结论]甘薯和枸杞更有利于维持水环境的稳定,蕹菜对硝酸盐、磷酸盐去除效果更好。

[Objective]To study the effects of Ipomoea aquatica,Ipomoea batatas and Lycium chinense on the microbial community structure in shrimp-vegetable symbiotic system.[Method]The composition of microbial community in shrimp-vegetable symbiotic system growing different vegetables was analyzed by high-throughput sequencing technique,and the indexes of nitrate and phosphate in the system were detected.[Result]The number of OTU shared by microorganisms in different shrimp-vegetable symbiotic systems was 450 species,among them,the number of OTU in Lycium chinense group(LM)was the highest(852 species),and the Shannon-Wiener index and Simpson index were the highest.The removal rates of nitrate and phosphate in Ipomoea aquatica(IF)were 65.70%and 67.57%,respectively,and the community structure was significantly different from other groups(P<0.05).At the gate level,the dominant flora in different systems were Proteobacteria,Actinobacteria and Patescibacteria.At the genus level,while the dominant flora in different systems were Rhodococcus,Acinetobacter and Glutamicibacter.[Conclusion]Ipomoea batatas and Lycium chinense were more beneficial to maintain the stability of water environment,and Ipomoea aquatica was more effective in removing nitrate and phosphate.