基于BSA-seq技术挖掘番茄耐裂果候选基因

Extraction of Tomato Cracking Tolerance Candidate Genes Based on BSA-seq Technique

[目的]明确番茄耐裂基因在染色体上的位置。[方法]以耐裂果番茄14FP5和易裂果番茄14FP26为试材,将二者杂交得到F 1代,F 1自交得到F 2,分别构建2个极端性状的DNA混合池,采用BSA-seq方法进行定位。[结果]利用BSA-seq对F 2群体的2个极端混池进行基因组重测序分析,将番茄耐裂基因定位在5号染色体上,位于34140000~38120000 pb,区间大小为3.98 Mb,候选区间内共注释到38个基因,其中非同义突变基因4个。[结论]该研究结果可为番茄耐裂基因的精细定位提供数据支撑,为番茄耐裂分子育种奠定基础。

[Objective]To determine the position of tomato crack tolerance gene on chromosome.[Method]Tomato 14FP5 which is resistant to cracking and tomato 14FP26 which is easy to crack are used as test materials,cross the two to get F 1 generation,F 1 self-cross to get F 2,DNA mixing pools with two extreme traits were constructed respectively,BSA-seq method was used to locate the location.[Result]BSA-seq was used for genome resequencing analysis of 2 extreme mixed pools of F 2 population.The tomato anti-cracking gene was initially located on chromosome 5,located between 34140000 and 38120000 pb,the interval size is 3.98 Mb,a total of 38 genes were annotated in the candidate region,including 4 non-synonymous mutant genes.[Conclusion]The results provide data support for the fine localization of tomato crack tolerance genes,and lay a foundation for tomato crack tolerance molecular breeding.