胃癌患者血清微小RNA-506和微小RNA-874的表达及其与幽门螺杆菌感染、病情严重程度的相关性

The expression levels of serum microRNA-506 and microRNA-874 in gastric cancer patients and their correlations with Helicobacter pylori infection and disease severity

目的探讨胃癌患者血清中微小RNA-506(miR-506)和微小RNA-874(miR-874)表达与幽门螺杆菌(Hp)感染及病情严重程度的相关性。方法选取2020年5月—2022年5月收治的78例胃癌患者为研究对象,根据Hp感染情况将患者分为感染组43例、未感染组35例。采用Pearson法分析患者血清miR-506与miR-874表达水平的相关性,采用Logistic回归分析探讨影响病情严重程度的相关因素,采用受试者工作特征(ROC)曲线分析miR-506、miR-874对Hp感染及病情严重程度的诊断效能。结果感染组患者血清miR-506水平为(1.41±0.33),高于未感染组的(1.20±0.28),差异有统计学意义(P<0.05);感染组血清miR-874表达水平为(1.22±0.27),低于未感染组的(1.50±0.51),差异有统计学意义(P<0.05)。Ⅲ~Ⅳ期患者血清miR-506和胃泌素-17(G-17)水平高于Ⅰ~Ⅱ期患者,miR-874和胃蛋白酶Ⅰ(PGⅠ)表达水平低于Ⅰ~Ⅱ期患者,差异有统计学意义(P<0.05)。血清miR-506与miR-874表达水平呈负相关(r=-0.886,P<0.05)。年龄、性别、肿瘤直径、分化程度与病情严重程度均无相关性(P>0.05)。Ⅲ~Ⅳ期患者淋巴结转移率高于Ⅰ~Ⅱ期患者,差异有统计学意义(P<0.05)。Logistic回归分析结果显示,miR-506、miR-874为病情严重程度的影响因素(P<0.05)。ROC曲线分析结果显示,血清miR-506、miR-874以及两者联合检测诊断Hp感染的曲线下面积(AUC)分别为0.741、0.648、0.823,两者联合检测优于血清miR-506和miR-874单独检测(P=0.007、0.001);血清miR-506、miR-874以及两者联合检测诊断病情严重程度的AUC分别为0.697、0.797、0.836,两者联合检测的预测效能优于血清miR-506和miR-874各自单独检测(P=0.045、0.046)。结论血清miR-506和miR-874表达水平与Hp感染的发生及病情严重程度有关,两者联合检测对Hp感染及病情严重程度具有一定的辅助诊断价值。

Objective To investigate the correlations of the expressions of microRNA-506(miR-506)and microRNA-874(miR-874)in serum of patients with gastric cancer with Helicobacter pylori(Hp)infection and severity of disease.Methods A total of 78 patients with gastric cancer admitted to our hospital from May 2020 to May 2022 were selected in the study,and the patients were divided into infection group(43 cases)and non-infection group(35 cases)according to Hp infection.Pearson method was applied to analyze the correlation between the expression levels of miR-506 and miR-874 in serum of patients;Logistic regression was used to analyze the related factors affecting the severity of the disease,and receiver operating characteristic(ROC)curve was used to analyze the diagnostic efficacy of miR-506 and miR-874 in Hp infection and severity of the disease.Receiver operating characteristic(ROC)curve was used to analyze the diagnostic efficacy of miR-506 and miR-874 in Hp infection and disease severity.Results The serum miR-506 level in the infection group was higher than that in the non-infection group,and the expression level of miR-874 was lower than that in the non-infection group(1.22±0.27 versus 1.50±0.51,P<0.05);the serum levels of miR-506 and gastrin-17(G-17)in patients of stageⅢtoⅣwere higher than those in patients of stageⅠtoⅡ,and the expression levels of miR-874 and pepsinⅠ(PGⅠ)were lower than those in patients of stage I toⅡ(P<0.05).The expression levels of miR-506 and miR-874 in serum were negatively correlated(r=-0.886,P<0.05).There were no correlations of age,sex,tumor diameter and differentiation degree with the severity of disease(P>0.05).The lymph node metastasis rate in patients with stageⅢtoⅣwas higher than that in patients with stageⅠtoⅡ(P<0.05).Logistic regression analysis showed that miR-506 and miR-874 were the influencing factors of severity of the disease(P<0.05).ROC curve analysis results showed that the area under the curve(AUC)of serum miR-506,miR-874 and their combined detection for diagnosis of Hp infection were 0.741,0.648 and 0.823,respectively.The combined detection was better than that of serum miR-506 and miR-874 alone(P=0.007,0.001).The AUC of serum miR-506,miR-874 and their combined detection for diagnosis of disease severity were 0.697,0.797 and 0.836,respectively.The predictive efficiency of the combined detection of the two methods was better than that of serum miR-506 and miR-874 separately(P=0.045,0.046).Conclusion The expression levels of miR-506 and miR-874 in serum are related to the occurrence and severity of Hp infection.The combined detection of the combined detection has certain auxiliary diagnostic value for Hp infection and severity of the disease.