茶树ARR-B基因家族的鉴定和表达模式分析

Identification and Expression Pattern Analysis ofARR-B Gene Family in Camellia sinensis

ARR-B基因作为细胞分裂素信号转导的正向调节因子,在植物发育及抗逆过程中发挥着重要作用。本研究基于茶树基因组数据库,利用生物信息学软件成功鉴定出23个茶树ARR-B基因,并对其基因结构、进化关系、保守结构域、染色体定位、启动子顺式作用元件以及八大组织和四种胁迫中的转录组数据等进行分析,并基于以上数据对福鼎大白茶树进行盐胁迫处理后利用荧光定量PCR技术验证表达水平。结果表明,茶树ARR-B基因家族有23个成员且在不同染色体上分布不均;根据系统进化树将茶树ARR-B基因分为9类且与拟南芥ARR-B基因同源性很高;茶树ARR-B基因相同亚家族的基因结构和保守结构域基本一致;茶树ARR-B基因在茎和根组织中具有较高的表达水平及在干旱、低温、盐和茉莉酸甲酯胁迫处理下存在差异表达;实时荧光定量PCR结果可知,经盐胁迫处理后,8个茶树ARR-B基因表达水平与转录组数据存在差异。本研究初步分析了茶树ARR-B基因家族的功能和抗逆作用,为进一步研究茶树的生长发育提供参考和依据。

As a positive regulator of cytokinin signal transduction,ARR-B gene plays an important role in plant development and stress resistance.This study was based on the Camellia sinensis genome database,using bioinformatics software to successfully identify 23 Camellia sinensis ARR-B genes,and their gene structure,evolutionary relationship,conserved domains,chromosome location,promoter cis-acting clements,and transcriptome data in eight major tissues and four stresses were analysed.Based on the above data,the Fuding Dabai Camellia sinensis was subjected to salt stress treatment and the expression level was verified by fluorescent quantitative PCR technology.The results showed that there were 23 members of the Camellia sinensis ARR-B gene family and they were unevenly distributed on different chromosomes;according to the phylogenetic tree,the Camellia sinensis ARR-B genes were divided into 6 categories and were homologous to the Arabidopsis thaliana.The gene structure and conserved domains of the same subfamily of the Camellia sinensis ARR-B gene were basically the same;The Camellia sinensis ARR-B gene had higher expression in stem and root tissues and under drought,low temperature,salt and methyl jasmonate stress.There were differential expressions under treatment.Real-time fluorescent quantitative PCR results showed that after salt stress treatment,the expression levels of ARR-B genes in 8 Camellia sinensis s were different from the transcriptome data.This study preliminarily analyzed the functions and anti-stress effects of the ARR-B gene family of Camellia sinensis,and provided reference and basis for further research on the growth and development of Camellia sinensis.