响应面法优化超声辅助提取荆芥中维生素C工艺

Optimization Technique of Ultrasonic-Assisted Extraction of Vitamin C from Catnip by RSM

采用响应面法对超声波辅助提取荆芥中维生素C的工艺进行研究。根据单因素试验结果,利用Central Composite Design原理进行响应面设计并进行优化。结果显示:当乙醇体积分数为70%,液料比为30:1(mL/g),超声温度为60℃,超声时间为30 min时,维生素C提取率24.39 mg/g,为最佳提取条件,而模型预测维生素C提取率为24.46mg/g,理论预测值与试验结果接近,说明该响应面模型有效可靠,为进一步开发利用香菜资源提供技术依据。

This paper studied the optimization technique of ultrasound-assisted extraction of catnip vitamin C by response surface methodology.According to single factor experimental results,the experiment was designed and optimized using the Central Composite Design.The results showed the optimal extraction condition was ethanol concentration 70%,the liquid material ratio 30∶1(mL/g),ultrasonic temperature 60℃,and ultrasonic time 30 min.Under the condition,the vitamin C extraction yield was 24.39 mg/g,and it was close with the value predicted from the theoretical model which was 24.46 mg/g.Antioxidant determination of extracts was carried out,which indicated that the response surface model was effective and reliable.The research provided certain theoretical basis for the industrial extraction of vitamin C from catnip.