高温胁迫对两个杉木种源多光谱荧光和抗氧化酶的影响

Effects of High Temperature Stress on Multispectral Fluorescence and Antioxidant Enzyme in Two Chinese Fir Provenances

本研究选择福建三明(FJSM)和江西九江(JXJJ)两地两年生的杉木种源作为试验材料,通过在人工气候箱内进行盆栽试验,研究了不同温度(25℃,30℃,35℃,40℃)对两个杉木种源的抗氧化酶活性和多光谱荧光的影响。结果表明:杉木的多光谱荧光参数F440、F520、F690、F740及其比值F690/F740随着高温胁迫的增加而升高。在高温胁迫下,FJSM的F690/F740的升高程度低于JXJJ。F690/F740的升高表明叶绿素含量的下降,FJSM种源在高温胁迫下叶绿素降解速度较慢,对高温的抗性更强。另外,经过高温胁迫后,杉木的SOD、POD和CAT等抗氧化酶活性总体上升。与JXJJ种源相比,FJSM在高温胁迫下抗氧化酶活性上升更大,下降趋势更小,表明FJSM更强的抗高温能力与抗氧化酶密切相关。

This study selected two-year old Chinese fir provenances from Sanming in Fujian Province(FJSM)and Jiujiang in Jiangxi Province(JXJJ)as experimental materials,and conducted pot experiments in an artificial climate box to study the effects of different temperatures(25℃,30℃,35℃,40℃)on the antioxidant enzyme activity and multispectral fluorescence of the two Chinese fir provenances.The results showed that the multispectral fluorescence parameters F440,F520,F690,F740 and their ratios F690/F740 of Chinese fir increased with the increase of high temperature stress.Under high temperature stress,the elevation of F690/F740 in FJSM was lower than that in JXJJ.The elevation of F690/F740 indicated a decrease in chlorophyll content,the slower chlorophyll degradation rate in FJSM under high temperature stress suggested a stronger resistance to high temperatures.In addition,after high temperature stress,the overall activity of antioxidant enzymes such as SOD,POD,and CAT in Chinese fir increased.Compared with the JXJJ source,FJSM showed a greater increase in antioxidant enzyme activity and a smaller decrease trend under high temperature stress,indicating that FJSM's stronger high-temperature resistance is closely related to antioxidant enzymes.