丙泊酚对高糖诱导的人肾小球系膜细胞氧化应激和炎症反应的影响

Effects of propofol on high glucose-induced oxidative stress and inflammatory responses in human mesangial cells

目的探讨丙泊酚对高糖诱导的人肾小球系膜细胞损伤的影响。方法该研究于2021年3月至2022年3月进行。体外培养人肾小球系膜细胞,采用30 mol/L葡萄糖诱导建立人肾小球系膜细胞细胞损伤模型,用丙泊酚浓度10、20、40μmol/L处理细胞,细胞分为对照组、高糖组、高糖+低、中、高剂量丙泊酚组。酶联免疫吸附测定(ELISA)检测超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、丙二醛、活性氧、白细胞介素(IL)-10、肿瘤坏死因子α(TNF-α)IL-1β表达;蛋白质印迹法检测诱导型一氧化氮合酶(iNOS)、细胞间黏附分子-1(ICAM-1)、单核细胞趋化蛋白-1(MCP-1)、IL-10、TNF-α、IL-1β蛋白表达。结果高糖组的丙二醛[(16.7±1.7)mmol/L比(3.8±0.4)mmol/L]、活性氧[(9.6±0.9)μg/L比(3.5±0.3)μg/L]、IL-10[(65.3±6.9)ng/L比(26.9±3.2)ng/L]、TNF-α[(105.6±10.9)ng/L比(42.8±4.8)ng/L]和IL-1β[(79.7±8.2)ng/L比(31.2±3.6)ng/L]明显高于对照组;iNOS、ICAM-1、MCP-1、IL-10、TNF-α、IL-1β蛋白表达明显高于对照组;SOD、GSH-Px明显低于对照组,均P<0.05。高糖+低剂量丙泊酚组、高糖+中剂量丙泊酚组、高糖+高剂量丙泊酚组的丙二醛、活性氧、IL-10、TNF-α和IL-1β明显低于高糖组;iNOS、ICAM-1、MCP-1、IL-10、TNF-α、IL-1β蛋白表达明显低于高糖组,均P<0.05;SOD、GSH-Px明显高于高糖组,均P<0.05。结论丙泊酚能减轻高糖诱导的人肾小球系膜细胞氧化应激和炎症反应,发挥保护肾脏的作用。

Objective To investigate the effect of propofol on human glomerular mesangial cell injury induced by high glucose.Meth⁃ods The study was conducted from March 2021 to March 2022.Human glomerular mesangial cells were cultured in vitro,and the 30 mol/L glucose concentration was used to induce a human glomerular mesangial cell injury model,and the cells were treated with propofol concentrations of 10,20,and 40μmol/L.The cells were divided into control(Con)group,high glucose(HG)group,high glucose+propofol low,medium and high dose(HG+propofol-L,HG+propofol-M,HG+propofol-H)groups.ELISA method to detect superoxide dismutase(SOD),glutathione peroxidase(GSH-PX),malondialdehyde,reactive oxygen species,interleukin-10(IL-10)the expression of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β);Western blotting method to detect the inducible type nitric oxide synthase(iNOS),intercellular adhesion molecule-1(ICAM-1),monocyte chemoattractant protein-1(MCP-1),IL-10,TNF-αand IL-1βprotein expression.Results HG group malondialdehyde[(16.7±1.7)mmol/L vs.(3.8±0.4)mmol/L],malondialdehyde[(9.6±0.9)μg/L vs.(3.5±0.3)μg/L],IL-10[(65.3±6.9)ng/L vs.(26.9±3.2)ng/L],TNF-α[(105.6±10.9)ng/L vs.(42.8±4.8)ng/L]and IL-1β[(79.7±8.2)ng/L vs.(31.2±3.6)ng/L]were significantly higher than those in Con group;iNOS,ICAM-1,MCP-1,IL-10,TNF-α,IL-1βthe expression of protein were significantly higher than that of Con group;SOD and GSH-Px were significantly lower than that of Con group(all P<0.05).The malondialdehyde,malondialdehyde,IL-10,TNF-αand IL-1βof the HG+propofol-L group,HG+propofol-M group,and HG+propofol-H group were significantly lower than those of the HG group;iNOS,ICAM-1,MCP-1,IL-10,TNF-α,IL-1βprotein expression was significantly higher than that of Con group(P<0.05);SOD and GSH-Px were significantly higher than that of the HG group,the rest are P<0.05.Conclusion Propofol can reduce the oxidative stress and inflammation of human glomerular mesangial cells induced by high glucose,and play a role in protecting the kidneys.