儿童抽动障碍与肠道菌群及炎症反应、免疫指标相关性研究

Correlation between tic disorder and intestinal flora,inflammatory response and immune indexes in children

目的探究儿童抽动障碍与肠道菌群及炎症反应、免疫指标相关性研究。方法选取连云港市第二人民医院2022年1月至2023年6月收治的69例儿童抽动障碍病儿作为试验组,并选取69例同期在该院体检的健康儿童作为对照组。对比两组小儿肠道菌群及炎症反应[白细胞介素-6(IL-6)、肿瘤坏死因子α(TNF-α)]、免疫指标[分化抗原(CD)3^(+)、CD4^(+)、CD8^(+)],同时分析试验组病儿美国耶鲁综合抽动严重程度量表(YGTSS)评分与肠道菌群、炎症反应和免疫指标的相关性。结果试验组病儿双歧杆菌水平低于对照组[(7.15±0.98)IgCFU/g比(9.24±0.85)IgCFU/g],大肠埃希菌水平高于对照组[(7.19±0.77)IgCFU/g比(6.29±0.81)IgCFU/g](均P<0.05);试验组病儿血清IL-6[(3.45±0.39)ng/L比(1.44±0.31)ng/L]和TNF-α[(10.24±1.31)ng/L比(5.43±1.29)ng/L]水平显著高于对照组(均P<0.05);试验组病儿CD3^(+)[(57.99±1.32)%比(62.08±1.27)%]和CD4^(+)[(41.87±1.23)%比(48.25±1.18)%]水平低于对照组,CD8^(+)[(36.77±1.39)%比(31.85±1.27)%]水平高于对照组(P<0.05);Pearson分析结果显示双歧杆菌水平、CD3^(+)和CD4^(+)水平与YGTSS评分呈负相关(r=−0.57、−0.66、−0.67,均P<0.05),大肠埃希菌水平、IL-6、TNF-α和CD8^(+)水平与YGTSS评分呈正相关(r=0.68、0.68、0.62、0.59,均P<0.05)。结论儿童抽动障碍病儿存在明显的肠道菌群及炎症反应、免疫指标异常,其病情程度与肠道菌群及炎症反应、免疫指标存在明显的相关性。

Objective To explore the correlation between tic disorder and intestinal flora,inflammatory response and immune indexes in children.Methods Sixty-nine children with tic disorder in The Second People's Hospital of Lianyungang from January 2022 to June 2023 were enrolled as experimental group,meanwhile,another 69 healthy children were set as control group.The comparison was conducted on intestinal flora,inflammatory response[interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)]and immune indicators(CD3^(+),CD4^(+),CD8^(+)).Then the correlation between Yale Global Tic Severity Scale(YGTSS)score and intestinal flora,inflammatory response and immune indexes in tic disorder children was analyzed.Results Compared with the control group,experimental group had smaller proportion of Bifidobacterium[(7.15±0.98)IgCFU/g vs.(9.24±0.85)IgCFU/g]and larger proportion of Escherichia coli[(7.19±0.77)IgCFU/g vs.(6.29±0.81)IgCFU/g](both P<0.05).Serum levels of IL-6[(3.45±0.39)ng/L vs.(1.44±0.31)ng/L]and TNF-α[(10.24±1.31)ng/L vs.(5.43±1.29)ng/L]were elevated in experimental group compared with control group(both P<0.05).A reduction in CD3^(+)[(57.99±1.32)%vs.(62.08±1.27)%]and CD4^(+)[(41.87±1.23)%vs.(48.25±1.18)%]along with an increase in CD8^(+)[(36.77±1.39)%vs.(31.85±1.27)%]were detected in experimental group compared with control group(all P<0.05).Pearson correlation analysis found that the proportion of Bifidobacterium,CD3^(+)and CD4^(+)levels were negatively correlated with the YGTSS score(r=−0.57,−0.66,−0.67,all P<0.05),and the proportion of Escherichia coli,IL-6,TNF-αand CD8^(+)were positively correlated with the YGTSS score(r=0.68,0.68,0.62,0.59,all P<0.05).Conclusion Children with tic disorder have obvious abnormal intestinal flora,inflammatory response,and immune indicators,and the severity of the disease is significantly correlated with intestinal flora,inflammatory response,and immune indicators.