LINC01503在喉鳞癌中表达增高并促进喉鳞癌进展

Up-Regulation of LINC01503 Promotes Progression of Laryngeal Squamous Cell Carcinoma

目的:探讨长链非编码RNA LINC01503在喉鳞癌中的表达及其促癌作用。方法:通过qRT-PCR的方法检测LINC01503在喉鳞癌组织及细胞中的表达水平,并分析其表达水平与临床参数的关系,通过CCK-8、克隆形成实验检测LINC01503对喉鳞癌细胞增殖能力的影响,通过Transwell小室迁移及侵袭实验检测LINC01503对细胞迁移及侵袭能力的影响,通过裸鼠动物模型进行体内实验,进一步验证LINC01503促进喉鳞癌进展的生物学作用。结果:LINC01503在喉鳞癌中表达增高,其表达水平与喉鳞癌患者的临床分期、组织病理分化程度及颈部淋巴结是否转移有关。体内体外研究证实,通过质粒转染过表达LINC01503,能够促进喉鳞癌细胞的增殖、迁移及侵袭能力,能够促进裸鼠体内荷瘤的生长,而敲低LINC01503的表达能获得相反的结果。结论:LINC01503在喉鳞癌中表达增高,作为促癌基因,能够促进喉鳞癌的增殖、迁移及侵袭能力等恶性表型的进展。

Objective:To investigate the expression of long non-coding RNA(LncRNA)LINC01503 in laryngeal squamous cell carcinoma(LSCC)and its role in promoting cancer progression.Methods:The expression level of LINC01503 in LSCC tissues and cells was detected by qRT-PCR,and its correlation with clinical parameters was analyzed.The effects of LINC01503 on the proliferation,migration,and invasion abilities of LSCC cells were assessed using CCK-8 and colony formation assays,Transwell chamber migration and invasion assays,respectively.A nude mouse xenograft model was established to further validate the biological function of LINC01503 in promoting LSCC progression.Results:LINC01503 was upregulated in LSCC,and its expression level was associated with clinical stage,tumor differentiation grade,and lymph node metastasis in LSCC patients.In vitro and in vivo experiments demonstrated that overexpression of LINC01503 promoted the proliferation,migration,and invasion abilities of LSCC cells and tumor growth in nude mice,while knockdown of LINC01503 expression led to the opposite effects.Conclusion:LINC01503 is upregulated in LSCC and acts as an oncogene to promote the progression of LSCC by enhancing cell proliferation,migration,and invasion.