黄连素调节卵清蛋白诱导哮喘小鼠的Th细胞分化

Berberine Regulates Ovalbumin to Induce Th Cell Differentiation in Asthmatic Mice

目的:评估黄连素干预对过敏性哮喘模型小鼠Th细胞分化和Notch信号通路的调节作用,及对肺组织损伤的修复情况。方法:利用卵清蛋白(OVA)诱导小鼠,低、高剂量黄连素和地塞米松干预后将小鼠分为对照组、模型组、黄连素低剂量组、黄连素高剂量组、地塞米松组。观察各组小鼠一般情况,HE染色观察肺组织炎症浸润,流式细胞术检测肺组织悬液中Th1、Th2、Th17细胞比例,ELISA检测肺泡灌洗液IFN-γ、IL-4、IL-17水平,Western-blot检测肺组织中Notch1和Hes1蛋白表达。结果:与对照组相比,模型组小鼠饮食饮水减少,精神焦躁,口鼻有粘液,肺组织出现明显炎症,Th1细胞比例、IFN-γ水平下降(P<0.05),Th2、Th17细胞比例、IL-4、IL-17水平增加(P<0.05),Notch1和Hes1蛋白表达增加(P<0.05)。与模型组相比,黄连素和地塞米松干预后小鼠饮食饮水增加,精神状态好转,口鼻粘液减少,肺组织炎症减少,Th1细胞比例、IFN-γ水平增加(P<0.05),Th2、Th17细胞比例,IL-4、IL-17水平下降(P<0.05),Notch1和Hes1蛋白表达减少(P<0.05)。结论:黄连素干预治疗过敏性哮喘模型,可通过调节Th亚型分化,抑制Notch1蛋白表达,缓解肺组织炎症损伤。

Objective:To investigate the regulation of berberine on Th cell differentiation and Notch signaling pathway in allergic asthmatic-model mice,as well as the repair of lung tissue injury.Methods:Mice were induced by OVA,and divided into control group,model group,low-dose berberine group,high-dose berberine group,and dexamethasone group after intervention with low and high doses of berberine and dexamethasone.The general conditions of the mice in each group were observed,HE staining was used to observe the inflammatory infiltration of lung tissue,flow cytometry was used to detect the proportions of Th1,Th2,and Th17 cells in lung tissue suspension,ELISA was used to detect the levels of IFN-γ,IL-4,and IL-17 in bronchoalveolar lavage fluid,and Western blot was used to detect the expression of Notch1 and Hes1 protein in lung tissue.Results:Compared with the control group,the mice in the model group had reduced food and water intake,anxiety,mucus in the nose and mouth,obvious inflammation in the lung tissue,decreased Th1 cell proportion and IFN-γlevel(P<0.05),increased Th2 and Th17 cell proportions,and IL-4 and IL-17 levels(P<0.05),and increased Notch1 and Hes1 protein expression(P<0.05).Compared with the model group,the mice in the berberine and dexamethasone intervention groups had increased food and water intake,improved mental state,reduced mucus in the nose and mouth,reduced lung tissue inflammation,increased Th1 cell proportion and IFN-γlevel(P<0.05),decreased Th2 and Th17 cell proportions,and IL-4 and IL-17 levels(P<0.05),and decreased Notch1 and Hes1 protein expression(P<0.05).Conclusion:Berberine intervention can alleviate lung tissue inflammatory damage in allergic asthma model by regulating Th subset differentiation and inhibiting Notch1 protein expression.