用于水环境Cu^(2+)检测的微生物电化学传感器运行模式探索及微生物菌落分析

Exploration of operation mode of microbial electrochemical sensor for Cu^(2+) detection in water environment and analysis of microbial colonies

探索不同运行模式下的微生物电化学传感器检测重金属离子时,在灵敏度、响应时间等方面的差异。考察MFC电化学传感器在恒定外电阻、开路和恒定外加偏压这3种模式下不同浓度的Cu^(2+)对传感器的电压抑制率或电流下降量。结果表明,Cu^(2+)浓度检测范围为0~10 mg·L^(−1)时,恒定外电阻模式下的Cu^(2+)对传感器的电压抑制率范围46.98%~80.12%,线性相关系数R2为0.9794;开路模式下的Cu^(2+)对传感器的电压抑制率范围为63.15%~89.12%,线性相关系数R2为0.9726;恒定外加偏压模式下,加入Cu^(2+),传感器的电流下降量范围1.2144~3.730 mA,线性相关系数R2为0.9476。开路模式下的MFC电化学传感器对Cu^(2+)的检测性能更优秀。这为利用微生物电化学传感器实时监测水质中的重金属含量提供了理论和试验基础,同时也为进一步研究利用微生物电化学传感器实现水中各种污染物的实时监测奠定了基础。

The differences in sensitivity and response time of microbial electrochemical sensors in different operating modes was explored for detecting heavy metal ions.In the operating modes of constant external resistance,open circuit and constant external bias,the voltage suppression rate or current drop of MFC electrochemical sensor was investigated with different concentration of Cu^(2+).The results showed that when the concentration of Cu^(2+)was 1~10 mg·L^(−1),the voltage suppression rate or current drop range to the sensor were 46.98%~80.12%,63.15%~89.12%and 1.2144~3.730 mA,respectively.And the linear correlation coefficients R2 were 0.9794,0.9726 and 0.9476,respectively.The MFC electrochemical sensor in open circuit mode had better detection performance for Cu^(2+).A theoretical and experimental basis has been provided for real-time monitoring of heavy metal in water by microbial electrochemical sensors.And a foundation for further research on real-time monitoring of various pollutants by microbial electrochemical sensors has been laid.