舒胃降逆颗粒对胃食管反流肝胃不和证的药效学考察

Pharmacodynamic study of Shuwei Jiangni Granules on hepato-stomach disharmony syndrome of gastroesophageal reflux

目的:研究舒胃降逆颗粒对胃食管反流肝胃不和证的疗效。方法:采用手术法建立胃食管反流大鼠模型,记录大鼠体质量变化、血清指标和病理组织学检测结果,观察舒胃降逆颗粒对胃食管反流的缓解作用;采用饥饱失常复合夹尾刺激法建立肝胃不和证大鼠模型,记录大鼠的一般状态、血清指标、胃蛋白酶活力和病理组织学检测结果,观察舒胃降逆颗粒对肝胃不和症状的缓解作用。结果:在胃食管反流型大鼠中,与假手术组比较,模型对照组大鼠的体质量以及胃动素(motilin,MTL)、胃泌素(gastrin,GAS)水平明显降低,TNF-α、IL-4以及NO水平均上升,且具有显著性差异;与模型对照组比较,舒胃降逆颗粒各剂量组大鼠的体质量以及MTL、GAS的水平均呈现上升趋势,TNF-α、IL-4以及NO水平均有下降趋势,食管下段病理组织黏膜层有一定程度的改善。在肝胃不和型大鼠中,与正常对照组比较,模型对照组大鼠的体质量、摄食量,以及MTL、GAS水平降低且具有显著性差异,5-HT、VIP以及胃蛋白酶的水平升高;与模型对照组比较,舒胃降逆颗粒各剂量组大鼠的体质量和摄食量,以及MTL、GAS水平均明显上升,5-HT、VIP以及胃蛋白酶水平均下降,存在显著性差异(P<0.05),胃部病理组织黏膜层有不同程度的改善。结论:舒胃降逆颗粒有缓解胃食管反流以及肝胃不和的作用,可以调节动物的免疫反应,对动物的情绪有一定的调节作用。

Objective To explore the curative effect of Shuwei Jiangni Granules(SJG)on hepato-stomach disharmony syndrome(HSDS)in gastroesophageal reflux disease(GERD).Methods The rat model of GERD was established by operation.The effect of SJG on alleviating GERD was observed by weight,serum parameters and histopathology.Rat model of HSDS was established by a combination of hunger and satiety disorder and tail clamp stimulation.The effect of SJG on alleviating HSDS was observed through the changes of general status,serum parameters,pepsin activity and histopathology.Results In rat model of GERD,as compared with sham-operation group,body weight and levels of motilin(MTL)and gastrin(GAS)declined markedly while the levels of tumor necrosis factor-alpha(TNF-α),interleukin-4(IL-4)and nitric oxide(NO)spiked obviously in model control group.As compared with model control group,body weight and level of MTL/GAS showed an upward trend while the levels of TNF-α,IL-4 and NO dropped.Mucosal layer of lower esophagus improved somewhat.In rat model of HSDS,as compared with normal control group,body weight,food intake and level of MTL/GAS of model control group declined markedly while the levels of 5-hydroxytryptamine(5-HT),vasoactive intestinal peptide(VIP)and pepsin spiked.As compared with model control group,body weight,food intake and levels of MTL/GAS showed a rising trend while the levels of 5-HT,VIP and pepsin declined obviously.Gastric mucosal layer improved somewhat.Conclusion SJG may relieve GERD/HSDS and regulate immune responses and emotions of animals.