小分子化合物G-749抑制胃癌细胞增殖和迁移

Small molecule compound G-749 suppresses the proliferation and migration of gastric cancer cells

目的 研究小分子化合物G-749对人胃癌细胞MGC803和SGC7901的增殖和迁移的影响。方法 分别用不同浓度的G-749处理MGC803和SGC7901两种人胃癌细胞,CCK8实验和克隆形成实验检测G-749对胃癌细胞增殖的影响,流式细胞术检测化合物对细胞周期的影响,划痕实验检测G-749对胃癌细胞迁移的影响。结果 化合物G-749能够抑制人胃癌细胞MGC803和SGC7901的增殖并存在剂量依赖关系,抑制细胞G_(0)/G_(1)期向S期的转变,并能明显抑制胃癌细胞的迁移。结论 化合物G-749对胃癌细胞有抑制作用,有望应用于胃癌细胞的治疗。

Objective eTo investigate the effects of small molecule compound G-749 on the proliferation and migration of human gastric cancer cells MGC803 and SGC7901.Methods MGC803 and SGC7901 human gastric cancer cells were treated with different concentrations of G-749.CCK8 assay and colony formation assay were used to detect the effect of G-749 on the proliferation of gastric cancer cells.Flow cytometry was used to check the effect of G-749 on the cell cycle.The scratch test was used to identity the effect of G-749 on the migration of gastric cancer cells.Results The compound G-749 inhibited the proliferation of human gastric cancer cells MGC803 and SGC7901 in a dose-dependent manner,and inhibited the transition of cells from G_(0)/G_(1) phase to S phase,and suppressed the migration of gastric cancer cells.Conclusion Compound G-749 has inhibitory effect on gastric cancer cells and is expected to be used in the treatment of gastric cancer cells.