苯乙醇及二氢茉莉酮对白纹伊蚊的驱避效果研究

The repellent effects of 2-phenylethanol and dihydrojasmone on Aedes albopictus

目的通过室内测试苯乙醇、二氢茉莉酮等19种木犀科和蔷薇科植物化合物的有效保护时间,探讨有效单体化合物及复配对白纹伊蚊的驱避效果。方法在实验室条件下,通过近距离反应测定法,测试体积比浓度为0.01的19种化合物对白纹伊蚊的驱避效果,选出驱避率最高的化合物进行复筛;通过Arm in cage行为实验,测试体积比浓度为0.064、0.128、0.256的苯乙醇、二氢茉莉酮对白纹伊蚊的驱避效果;将体积比浓度为0.256的苯乙醇和二氢茉莉酮,按照1∶1、1∶4、4∶1、1∶7、7∶1、1∶15、15∶1的比例两两混合,测试对白纹伊蚊的驱避效果;在0.256体积比浓度,以体积比为1∶15和15∶1的苯乙醇与二氢茉莉酮作为主要有效成分制备成驱蚊液,测试对白纹伊蚊的驱避效果。不同化合物对白纹伊蚊驱避作用的比较采用独立样本t检验和单因素方差分析。结果苯乙醇、二氢茉莉酮初筛驱避率均达到100%;0.064、0.128、0.256体积比浓度下的苯乙醇、二氢茉莉酮单独使用时对白纹伊蚊有驱避作用,与对照组比较有效保护时间差异均有统计学意义(均P<0.001);体积比浓度为0.256时,不同体积配比苯乙醇和二氢茉莉酮的驱避时间差异有统计学意义(F=21.457,P<0.001)。苯乙醇与二氢茉莉酮以1∶15和15∶1体积配比制备成的驱蚊液有效保护时间分别达到4.50和6.25 h。结论苯乙醇与二氢茉莉酮复配对白纹伊蚊具有明显驱避效果,可进一步研发应用。

Objective To investigate the repellent effects of 19 botanical compounds derived from Oleaceae and Rosaceae on Aedes albopictus by testing their effective protection time alone and in combination in laboratory.Methods Under laboratory conditions,the selected compounds showed the highest repellency rates against Ae.albopictus at a volume concentration of 0.01 among 19 compounds through the close proximity response assay.Next,2-phenylethanol and dihydrojasmone were tested using the Arm in cage test for their repellency to Ae.albopictus at volume concentrations of 0.064,0.128,and 0.256.Then 2-phenylethanol(concentration,0.256)and dihydrojasmone(concentration,0.256)were mixed at volume ratios of 1∶1,1∶4,4∶1,1∶7,7∶1,1∶15,and 15∶1 for Ae.albopictus repellency testing.Finally,the mosquito repellents of 2-phenylethanol(concentration,0.256)and dihydrojasmone(concentration,0.256)were prepared and tested at volume ratios of 1∶15 and 15∶1 as the main effective components.The repellent activity of different compounds or combinations against Ae.albopictus was compared by using the independent samples t-test and one-way analysis of variance.Results The repellent rates of 2-phenylethanol and dihydrojasmone both reached 100% by close proximity response assay.When used alone,2-phenylethanol and dihydrojasmone showed repellent effects on Ae.albopictus at concentrations of 0.064,0.128,and 0.256,and their effective protection time differed significantly from that of the control group(all P<0.001).At the concentration of 0.256,the repelling time was significantly different between the mixtures of 2-phenylethanol and dihydrojasmone at different volume ratios(F=21.457,P<0.001).For repellents with 2-phenylethanol and dihydrojasmone mixed at 15∶1 and 1∶15,the effective protection time reached 4.50 h and 6.25 h,respectively.Conclusion The combination of 2-phenylethanol and dihydrojasmone shows significant repellent effects on Ae.albopictus,which can be further developed.