超声定位显微成像对肿瘤演进及磁热疗效的评估

Evaluation of tumor progression and magnetic hyperthermia therapy efficacy with ultrasound localization microscopy

肿瘤的演进和消融伴随着微血管网络的变化,因此使用超声定位显微成像技术评估肿瘤区域微血管及其在磁热疗作用下的变化。通过构建小鼠肿瘤动物模型,搭建实验平台进行超声动物实验,实现肿瘤内部细微血管成像。通过向肿瘤部位注射磁纳米粒子,在外部磁场的作用下产生热效应,实现对肿瘤的磁热治疗。实验结果表明,超声定位显微镜成像能观察到肿瘤生长演进过程中微血管网络的变化,且其变化情况与免疫组化结果具有一致性。此外,磁热治疗后肿瘤内部血管明显减少,与病理切片结果相符,验证了磁热治疗的可行性。

Tumor progression and ablation are accompanied by changes in the microvascular network.Ultrasound localization microscopy(ULM)is a promising tool for observing such changes.This study aims to use this method to evaluate the microvasculature in tumor regions and its changes under magnetic hyperthermia treatment.By establishing a mouse tumor animal model and setting up an experimental platform for ultrasound experiments,imaging of the intratumoral microvasculature is achieved.Magnetic hyperthermia treatment combines an external magnetic field and injected magnetic nanoparticles,which causes heat effect on the tumor tissue.Experiment results show that the changes of intratumoral microvasculature during tumor evolution can be observed by the ULM,which was consistent with the results of immunohistochemistry.Furthermore,comparing the microvascular images of the tumor area before and after magnetic hyperthermia treatment,a significant reduction in blood vessels is found,which is also consistent with the pathological slice results.The results verified the feasibility of magnetic hyperthermia treatment.