黏细菌代谢产物对人神经胶质瘤的作用

Effect of myxobacteria metabolites on glioma

目的 对内蒙古自治区鄂尔多斯地区部分黏细菌进行分离纯化,检测其对人神经胶质瘤细胞U87的抑制作用,并鉴定其代谢产物类型。方法 采用滤纸片诱导法、大肠埃希菌划线法、兔粪诱导法,对内蒙古自治区鄂尔多斯地区采集的黏细菌土样进行分离纯化,将纯化菌株进行发酵培养,采用CCK-8法检测其发酵液对U87细胞的抑制率;采用化学显色法对菌株代谢产物进行初步定性分析。结果 分离纯化获得16株黏细菌,属于黏细菌的5个属,其发酵上清液对U87细胞均无抑制作用,大孔树脂甲醇浸提物对U87细胞均存在显著抑制效果,除E19菌株外,各菌株最高抑制率均值可达80%;不同种属菌株次级代谢产物类型差异明显,其中,珊瑚球菌属(Corallococcus)的代谢产物包含种类最多,对细胞抑制作用最强,抑制率达97.41%。结论 黏细菌的天然产物可作为抗神经胶质瘤的新型药源进行深入研究,相关工作可为抗癌新药的研发提供参考。

Objective To isolate and purify some myxobacteria from Ordos,Inner Mongolia Autonomous Region,detect the inhibitory effect of their metabolites on human glioma cell line U87,and identify the types of the metabolites.Methods Using filter paper induction method,E.coli marking method and rabbit feces induction method,the soil samples of myxo⁃bacteria collected in Ordos area of Inner Mongolia Autonomous Region were isolated and purified.The purified strains were fermented,the inhibition rate of the fermentation broth on U87 cells was detected by CCK⁃8 assay,and the metabolites were qualitatively analyzed by chemical chromogenic method.Results Sixteen strains of myxobacteria were isolated and puri⁃fied,belonging to five genera of myxobacteria.The fermentation supernatant had no inhibitory effect on U87 cells,while the methanol extract of macroporous resin had significant inhibitory effect on U87 cells.Except strain E19,the average highest inhibitory rate of all strains was 80%.There were significant differences in the types of secondary metabolites among different species of strains,among which the metabolites of Corallococcus contained the most species and exhibited the strongest inhibitory effect on the cells,with an inhibition rate of 97.41%.Conclusion The natural products of myxobac⁃teria can be used as a new anti⁃glioma drug source for in⁃depth study,and the related work can provide a reference for the development of new anticancer drugs.