血清miR-665和miR-302e在卵巢癌早期诊断中的临床应用

Clinical application of serum miR-665 and miR-302e in early diagnosis of ovarian cancer

目的探讨血清微小RNA(miR)-665和miR-302e表达在卵巢癌早期诊断中的临床应用。方法于2020年3月至2022年12月选取在中国人民解放军空军军医大学第二附属医院接受治疗的90例卵巢癌患者(卵巢癌组)及85例健康体检者(对照组),实时荧光定量聚合酶链反应(qRT-PCR)检测血清miR-665和miR-302e表达;Pearson法、ROC曲线、Logistic回归进行分析血清miR-665和miR-302e水平的相关性、血清中两者水平对卵巢癌的诊断价值以及影响卵巢癌发生的因素,并分析血清miR-665和miR-302e水平与卵巢癌患者临床病理的关系。结果与对照组相比,卵巢癌组患者血清中miR-665和miR-302e水平均显著下降,且差异有统计学意义(P<0.05);卵巢癌患者血清miR-665和miR-302e水平呈正相关(P<0.001);血清miR-665、miR-302e单独诊断卵巢癌患者的曲线下面积、敏感度、特异度分别为0.779、77.78%、70.59%,0.877、72.22%、87.06%,最佳截断值分别为0.960、0.880;二者联合诊断卵巢癌的曲线下面积为0.900,敏感度为67.78%,特异度为96.47%。Logistic回归分析显示,卵巢癌患者发生的影响因素为血清miR-665和miR-302e水平(P<0.05)。结论血清miR-665和miR-302e表达可用于卵巢癌早期诊断,且联合诊断效果更佳。

Objective To explore the clinical application of serum miRNA(miR)-665 and miR-302e expression in the early diagnosis of ovarian cancer.Methods From March 2020 to December 2022,90 ovarian cancer patients(ovarian cancer group)and 85 healthy individuals(control group)who received treatment in The Second Affiliated Hospital of the Chinese People’s Liberation Army Air Force Medical University were collected,Real-time quantitative fluorescence polymerase chain reaction(qRT-PCR)was applied to detect the expression of miR-665 and miR-302e in serum.Pearson method,receiver operating characteristic and Logistic regression were applied to analyze the correlation between serum miR-665 and miR-302e levels,the diagnostic value of the two levels in serum for ovarian cancer,and the factors affecting the occurrence of ovarian cancer,the relationship between serum miR-665 and miR-302e levels and clinical pathology of ovarian cancer patients was analyzed.Results Compared with the control group,the serum levels of miR-665 and miR-302e in the ovarian cancer group showed a statistically obvious decrease(P<0.05).There was a positive correlation between serum miR-665 and miR-302e levels in ovarian cancer patients(P<0.001).The area under the curve,sensitivity,and specificity of serum miR-665 and miR-302e in the diagnosis of ovarian cancer patients were 0.779,0.877,77.78%,72.22%,70.59%,and 87.06%,respectively,the optimal cutoff value was 0.960 and 0.880,respectively.The area under the curve for the combined diagnosis of ovarian cancer was 0.900,with a sensitivity of 67.78%and a specificity of 96.47%.Logistic regression analysis showed that the influencing factors for the occurrence of ovarian cancer in patients were serum levels of miR-665 and miR-302e(P<0.05).Conclusion The expression of miR-665 and miR-302e in serum can be used for early diagnosis of ovarian cancer,and the combined diagnosis is more effective.