双着丝粒染色体半自动分析剂量-效应曲线建立与验证

Establishment and validation of a dose-response curve for semi-automatic analysis of dicentric chromosomes

目的建立基于染色体全自动扫描分析系统的双着丝粒染色体(DC)半自动分析的剂量-效应曲线。方法采用X射线对3名健康志愿者的周围血进行照射,照射剂量分别为0.00、0.10、0.25、0.50、0.75、1.00、2.00、3.00、4.00和5.00 Gy,吸收剂量率为1.0 Gy/min。采用高通量染色体全自动分析系统对DC样本进行中期分裂相图像采集,通过DCScore软件自动分析DC畸变,人工确认后拟合DC半自动分析剂量-效应曲线。采用本实验室参加全国生物剂量估算能力比对的3份考核样品对拟合的DC半自动分析剂量-效应曲线进行准确性验证。结果在0.00~5.00 Gy剂量范围内,DC率随着照射剂量的增加而增加(P<0.01)。拟合的DC半自动分析的剂量-效应曲线为y=0.0008(±0.0002)+0.0092(±0.0009)D+0.0142(±0.0004)D^(2)(决定系数为0.9998)。对3份考核样品估算剂量与实际剂量相对偏差在20.00%左右,可以用于生物剂量估算;且除去与人工分析相同操作的使用时间,半自动分析方法可将分析效率提高26.0倍。结论构建X射线诱导的DC半自动分析剂量-效应关系曲线进行生物剂量估算,可数倍缩短人工分析时间,在大规模辐射事故的核应急响应中具有很大的应用前景。

Objective To establish a dose-effect curve for semi-automatic analysis of dicentric chromosomes(DC)based on an automatic chromosome analysis system.Methods A total of three healthy volunteers were recruited as the study subjects,and their peripheral blood was collected and stimulated by X-ray at doses of 0.00,0.10,0.25,0.50,0.75,1.00,2.00,3.00,4.00,and 5.00 Gy,with the absorbed dose rate of 1.0 Gy/min.Images of DC in the mid-stage of cell division were collected using a high-throughput automatic chromosome analysis system.The DCScore software was used to automatically analyze DC aberrations,and a dose-effect curve for semi-automatic analysis of DC was fitted after manual confirmation.The fitted dose-effect curve for semi-automatic analysis of DC was validated for accuracy using three proficiency test samples from the national quality assessment of biological dose.Results The incidence of DC increased with increasing irradiation doses in the range of 0.00-5.00 Gy(P<0.01).The dose-effect curve for the fitted semi-automatic analysis of DC was y=0.0008(±0.0002)+0.0092(±0.0009)D+0.0142(±0.0004)D^(2)(R^(2)=0.9998).The relative deviation between the estimated dose and the actual dose of the three test samples was about 20.00%,indicating curve applicability for biological dose estimation.Moreover,excluding the time spent on manual analysis,the semi-automatic analysis method increased the analysis efficiency by 26.0 times.Conclusion The semi-automatic analysis dose-effect curve for DC stimulated by X-ray is constructed for biological dose estimation,which can reduce the manual analysis time,and holds great potential for application in nuclear emergency response to large-scale radiation accidents.