伪狂犬病病毒感染猪脾淋巴细胞的转录组学分析

Transcriptomic Analysis of Porcine Spleen Lymphocytes Infected with Pseudorabies Virus

旨在探究伪狂犬病病毒(PRV)体外感染猪脾淋巴细胞基因组转录水平的变化,通过检测PRV感染猪脾淋巴细胞组蛋白乙酰化酶(HATs)、组蛋白去乙酰化酶(HDACs)的基因表达水平,筛选感染关键时间点进行PRV感染细胞的高通量测序,分析PRV感染后基因表达层面的变化。结果表明,PRV感染后共筛选出2293个差异基因(DEGs),其中有726个基因表达上调,1567个基因表达下调。GO富集分析显示DEGs广泛分布于细胞膜、细胞核和细胞质中,参与调节细胞膜受体活性、信号转导和免疫等生物学进程。KEGG分析发现差异基因主要富集到免疫相关信号通路,如Toll样受体信号通路、NOD样受体信号通路等,提示PRV致病机制可能与炎症反应和细胞凋亡有关,研究结果可为进一步了解PRV感染猪免疫系统的分子机制提供重要参考。

The aim of this study is to investigate the changes in the genomic transcription levels of porcine splenic lymphocytes infected with pseudorabies virus(PRV)in vitro,in order to provide a theoretical basis for the development of drugs for the prevention and treatment of PRV.In this study,we detected the gene expression levels of histone acetyltransferases(HATs)and histone deacetylases(HDACs)in swine spleen lymphocytes infected with PRV,and screened for critical time points of infection for high-throughput sequencing analysis to analyze the changes in gene expression at the level of PRV infection.The results showed that a total of 2293 differentially expressed genes(DEGs)were identified after PRV infection,including 726 up-regulated genes and 1567 down-regulated genes.GO enrichment analysis showed that DEGs were widely distributed in the cell membrane,nucleus and cytoplasm,participating in the regulation of cellular membrane receptor activity,signal transduction,immunology,and other biological processes.KEGG analysis found that the differentially expressed genes were mainly enriched in immune-related signaling pathways,such as the Toll-like receptor signaling pathway,NOD-like receptor signaling pathway,indicating that the pathogenic mechanism of PRV may be associated with inflammation and cell apoptosis,providing important reference for further understanding the molecular mechanism of PRV infection in pig immune organs.