硫自养-异养协同反硝化脱氮技术研究

Study on advanced denitrification technology of sulfur-based autotrophic denitrification

研究水力停留时间、进水硝酸盐浓度、pH值对硫自养反硝化脱氮的影响以及外加有机碳源构建的自养-异养反硝化系统的脱氮效能。结果显示:硫自养的优化工艺条件为进水硝酸盐浓度30 mg/L,水力停留时间2 h,pH值7,此时的硝酸盐去除率可达97.23%,容积负荷可达0.35 kg/(m^(3)·d)。适量增加碳源形成硫自养-异养反硝化体系时,硝酸盐去除率可达99.59%,容积负荷可达0.52 kg/(m^(3)·d),表明硫自养和异养反硝化具有协同作用。变形菌门是参与硫自养-异养协同反硝化过程的主要菌门,约占85.33%。铁氧化菌属是参与反硝化过程的主要菌属。

The effects of hydraulic retention time,influent nitrate concentration,and pH value on the denitrification of sulfur autotrophic denitrification and the denitrification efficiency of the autotrophic and heterotrophic denitrification system constructed by adding organic carbon sources were studied.The results showed that the optimal process conditions for sulfur autotrophic were influent nitrate concentration of 30 mg/L,hydraulic retention time of 2 h,pH value of 7,nitrate removal rate of 97.23%,and volume load of 0.35 kg/(m^(3)·d).When the carbon source is increased to form a sulfur autotrophic and heterotrophic denitrification system,the nitrate removal rate can reach 99.59%,and the volume load can reach 0.52 kg/(m^(3)·d),indicating that sulfur autotrophic and heterotrophic denitrification have a synergistic effect.Proteobacteria are the main phylum involved in the process of sulfur autotrophic and heterotrophic synergistic denitrification,accounting for about 85.33%.Iron oxidizing bacteria are the main genus involved in the denitrification process.