牛肉中苯甲酸含量分析

Analysis of Benzoic Acid Content in Beef

采用液相色谱法对1660个牛肉样本中的苯甲酸含量进行测定,结果显示,含量小于定量限(10 mg/kg)的样本占94.7%,其他5.3%样本中苯甲酸含量大于20 mg/kg。为进一步确定牛肉中苯甲酸含量,采用超高效液相色谱-串联质谱法对从屠宰场和批发市场采集的390个牛肉样本中的苯甲酸及其代谢物马尿酸含量进行测定,其中50个确定来源的牛肉样本中苯甲酸含量小于0.20 mg/kg。应用统计学分析手段获取牛肉中苯甲酸及马尿酸含量分布结果,结果表明:收集的340个牛肉中苯甲酸检出率为62.4%(大于12.5μg/kg),含量范围为未检出至131.41 mg/kg,平均值2.75 mg/kg,中位数0.10 mg/kg;其代谢物马尿酸检出率为100.0%,含量范围5.66×10^(-3)~1.15 mg/kg,平均值0.15 mg/kg,中位数0.10 mg/kg。此外,结合文献报道及苯甲酸含量数据对牛肉中苯甲酸进行风险分析,发现非法“注水”行为是导致牛肉中苯甲酸残留量较高的重要风险来源之一。

The content of benzoic acid in 1660 beef samples was determined by liquid chromatography(LC).The result showed that the content of benzoic acid was lower than the limit of quantification(10 mg/kg)in 94.7%of the samples and was greater than 20 mg/kg in the other 5.3%.The levels of benzoic acid and its metabolite hippuric acid in 390 beef samples obtained from slaughterhouses and wholesale markets were determined by ultra-high performance liquid chromatographytandem mass spectrometry(UPLC-MS/MS).The benzoic acid content in 50 confirmed beef samples was less than 0.20 mg/kg.The statistical analysis of the distribution of benzoic acid and hippuric acid in beef indicated that the detection rate of benzoic acid in 340 samples was 62.4%(greater than 12.5μg/kg),and the content of benzoic acid ranged from nondetectable to 131.41 mg/kg,with an average value of 2.75 mg/kg and a median value of 0.10 mg/kg.The detection rate of hippuric acid was 100.0%,and its content ranged from 5.66×10^(-3) to 1.15 mg/kg,with an average value of 0.15 mg/kg and a median value of 0.10 mg/kg.Furthermore,risk analysis of benzoic acid residue in beef was carried out based on literature data and the benzoic acid content data obtained,revealing that water injection could be an important risk source that causes high benzoic acid residue levels in beef.