不同部位东南五味子的挥发油成分及体外药理活性研究

Study on Volatile Oil Components and In Vitro Pharmacological Activity of Different Parts of Schisandra henrgi subsp.marginalis

目的:对比研究不同部位东南五味子挥发油成分,并探究其体外药理活性。方法:采用水蒸气蒸馏法提取东南五味子叶、茎、根挥发油,经GC-MS技术分离和鉴定不同部位挥发油,利用归一化法测定其相对百分含量。通过ABTS测定抗氧化活性,采用Griess法检测细胞培养液中NO的含量,比较不同部位东南五味子挥发油的抗氧化活性和抗炎活性。结果:共鉴定出46个成分。东南五味子叶、茎、根分别鉴定出33、32、29个成分,分别占各自挥发油总量的93.01%、89.15%、88.90%;东南五味子根、茎、叶抗氧化测定结果的IC_(50)值分别0.339、0.175、1.111 mg/mL,均有较好的抗氧化能力,抗炎测定结果显示东南五味子叶处理组的细胞培养液中NO的含量均显著降低(P<0.05),且随着剂量的增加,NO含量有减少的趋势。结论:东南五味子挥发油成分种类主要为醇类和烯烃类,不同部位挥发油成分及相对百分含量具有一定差异,但均有较好的抗氧化活性,且叶具有抗炎活性。

Objective:To study the components of volatile oil from different parts of Schisandra henrgi subsp.marginalis and to explore their pharmacological activities in vitro.Methods:The essential oils of the leaves,stems and roots of Schisandra henrgi subsp.marginalis were extracted by steam distillation.The essential oils of different parts of Schisandra henrgi subsp.marginalis were isolated and identified by GC-MS technique.The relative percentage of the essential oils was determined by normalization method,the antioxidant activity of different parts of Schisandra henrgi subsp.marginalis was determined by ABTS method,and NO content in cell culture medium was detected by Griess method.The antioxidant and anti-inflammatory activities of volatile oils from different parts of Schisandra henrgi subsp.marginalis were compared.Results:A total of 46 components were identified.33,32 and 29 components were identified from the leaves,stems and roots of Schisandra henrgi subsp.marginalis,which accounted for 93.01%,89.15%and 88.90%of the total volatile oil,respectively.The IC50 values of the antioxidant test results of the roots,stems and leaves of Schisandra henrgi subsp.marginalis were 0.339,0.175 and 1.111 mg/mL,respectively,all of which showed good antioxidant capacity.The results of anti-inflammatory assay showed that NO content in 12.5,6.25,3.125,1.5625 and 0.78125μg/mL cell culture medium of Schisandra henrgi subsp.marginalis leaves groups was significantly decreased(P<0.05),and the NO content showed a tendency to decrease with the increase of dose.Conclusion:The components of the volatile oil of Schisandra henrgi subsp.marginalis are mainly alcohols and olefins,and the components and relative contents of the volatile oil in different parts are different to some extent,showing better antioxidant activities,and leaves show the anti-inflammatory activity.