硫酸新霉素在黑脊倒刺鲃(Spinibarbus caldwelli)体内的残留消除规律研究

Studies on the residues and degradation rules of neomycin sulfate in Spinibarbus caldwelli

在(23±2)℃水温条件下,按黑脊倒刺鲃(Spinibarbus caldwelli)体质量60 mg/kg单剂量混饲口灌硫酸新霉素后,采用液质联用法测定肌肉、肝脏和肾脏中的药物浓度,研究硫酸新霉素在黑脊倒刺鲃体内的残留消除规律。结果表明,黑脊倒刺鲃单剂量口灌硫酸新霉素后,硫酸新霉素在黑脊倒刺鲃肌肉、肝脏和肾脏中的消除半衰期T 1/2ke分别为44.62、248.50和616.90 h,说明硫酸新霉素在肌肉中消除最快,在肝脏中消除缓慢,在肾脏中消除最慢。以可食用的肌肉作为残留检测的靶组织,根据现行的GB 31650—2019《食品安全国家标准食品中兽药最大残留限量》规定,新霉素在所有食品动物肌肉中的最高残留限量为500μg/kg,建议在本试验条件下黑脊倒刺鲃至少在给药10 d后才可食用。

At a water temperature of(23±2)℃,Spinibarbus caldwelli was treated with a single oral administration of neomycin sulfate at a dosage of 60 mg/kg body weight.The concentration of neomycin sulfate in the muscle,liver,and kidney of Spinibarbus caldwelli was determined using HPLC-MS/MS,and the residues and degradation patterns of neomycin sulfate in Spinibarbus caldwelli were studied.The results revealed that after a single dose of oral administration of neomycin sulfate,the elimination half-life T 1/2ke of neomycin sulfate in the muscle,liver,and kidney of Spinibarbus caldwelli were 44.62 h,248.50 h,and 616.90 h,respectively.This indicated that neomycin sulfate was eliminated most rapidly in the muscle,followed by the liver,and slowest in the kidney.Muscle was used as the target tissue for residual detection.According to the current National Food Safety Standard-Maximum Residue Limit of Veterinary Drugs in Food(GB 31650—2019),the maximum residue limit of neomycin sulfate in all food animal muscles is set at 500μg/kg.Therefore,it is recommended to refrain from consuming Spinibarbus caldwelli until at least 10 d have passed under the experimental conditions to ensure safety.