NF-κB介导VEGF-A促进食管鳞状细胞癌血管生长和细胞增殖的实验研究

NF-κB mediates VEGF-A to promote vascular growth and cell proliferation in oesophageal cancer

目的:探讨NF-κB、VEGF-A在食管鳞状细胞癌(ESCC)患者癌组织中的表达情况及NF-κB与VEGF-A在促进食管鳞癌血管生成和细胞增殖的关系。方法:收集32例行食管癌切除术患者食管鳞癌组织及癌旁组织标本,蛋白印迹法(Western blot)检测癌及癌旁组织中NF-κB、VEGF-A的蛋白表达情况;Pearson相关系数分析NF-κB与VEGF-A的相关性。在体外实验中过表达和敲低食管鳞癌细胞中的NF-κB后,通过CCK-8、平板克隆实验、流式细胞术检测NF-κB对肿瘤细胞恶性表型的影响。Western blot检测不同NF-κB表达水平对VEGF-A及VEGF-R2的表达水平的变化。结果:NF-κB、VEGF-A在癌组织中高表达,在癌旁组织中低表达。过表达和敲低NF-κB对细胞的增殖分别有促进和抑制作用、对细胞的凋亡有抑制和促进作用、对细胞周期的G1/S转换有促进和抑制作用。过表达和敲低NF-κB后VEGF-A、VEGF-R2的表达也随之升高和降低。结论:ESCC组织中的NF-κB与VEGF-A表达高于癌旁组织,二者正相关,且NF-κB通过过表达VEGF-A对ESCC血管生成和细胞增殖具有正向促进作用。

Objective:To explore the expression of NF-κB and VEGF-A in patients with esophageal squamous cell carcinoma(ESCC),the relationship between NF-κB and VEGF-A in promoting angiogenesis and cell proliferation in ESCC.Methods:A total of 32 specimens of esophageal squamous cell carcinoma tissues and adjacent tissues were collected from patients who underwent esophageal cancer resection.Western blot analysis was used to assess the protein expression of NF-κB and VEGF-A in cancerous and adjacent tissues,and the correlation between NF-κB and VEGF-A was analyzed by the Pearson correlation coefficient.In vitro experiments involved the successful knockdown or overexpression of NF-κB in esophageal squamous cell carcinoma cells,followed by the examination of its effects on the malignant phenotype of tumor cells through CCK-8,plate cloning,and flow cytometry experiments.Western blot experiments were also conducted to detect changes in VEGF-A and VEGF-R2 expression levels associated with altered NF-κB expression.Results:NF-κB and VEGF-A was highly expressed in cancer tissues and low expressed in adjacent tissues.Overexpression and knockdown of NF-κB had promoting and inhibitory effects on cell proliferation,inhibitory and promoting effects on cell apoptosis,and promoting and inhibiting effects on G1/S transition of the cell cycle.VEGF-A and VEGF-R2 expression levels increased or decreased in accordance with the overexpression or knockdown of NF-κB.Conclusion:The expression of NF-κB and VEGF-A is higher in ESCC tissues compared to adjacent tissues,and there is a positive correlation between the two.NF-κB positively promotes angiogenesis and cell proliferation in ESCC through the upregulation of VEGF-A.