2种不同砧木对遵义红花椒麻味和挥发性物质的影响

The Effects of Two Different Rootstocks on the Sesame Flavor and Volatile Compounds of Zunyi Red ZanthoxylumBungeanum

为探讨2种不同砧木对遵义红花椒麻味和挥发性物质的影响。本研究采用高效液相色谱(HPLC)、固相微萃取(SPME)结合气相色谱质谱联用仪(GC-MS)的方法,检测不同样品中酰胺总含量、挥发性物质种类和相对含量。结果表明,2种不同砧木嫁接提高了遵义红花椒酰胺总含量,增加了遵义红花椒的麻味;不同样品中共鉴定出49种挥发性物质,主要为烯烃类、醇类和酯类,其中烯烃类含量最高。嫁接后遵义红花椒中烯烃类物质减少,醇类和酯类物质增加;柠檬烯、β-月桂烯、香桧烯和α-蒎烯含量减少,大牻牛儿烯-D、乙酸松油酯、桉叶脑和α-松油醇含量增加。本研究为遵义红花椒的繁育以及嫁接砧木的选择提供理论依据。

In order to explore the effects of two different rootstocks on the numb taste substances and volatile substances of Zunyi red Zanth-oxylum bungeanum.This article used high-performance liquid chromatography(HPLC),solid-phase microextraction(SPME)combined with gas chromatography-mass spectrometry(GC-MS)to detect the total amide content,volatile substance types,and relative content in dif-ferent samples.The results showed that the grafting of two different rootstocks increased the total content of pyruvamide in Zunyi red Zanth-oxylum bungeanum,and increased the numbness of Zunyi red Zanthoxylum bungeanum;A total of 49 volatile substances were identified in different samples,mainly olefins,alcohols,and esters,with the highest content of olefins.After grafting,the olefins in Zunyi red Zanth-oxylum bungeanum decreased,while the alcohols and esters increased;The of conter lemonene,-laurene,coumarinene,and-pinene de-creases,while the content of geranium-D,terpinyl acetate,eucalyptus and-pine oil alcohol has increased.This study provides a theoretical basis for the breeding of Zunyi red Zanthoxylum bungeanum and the selection of grafting rootstocks.