基于ICP-MS对三七血伤宁散中16种无机元素的分析及质量评价

Analysis and Quality Evaluation of 16 Inorganic Elements in Sanqi Xueshangning Powder Based on ICP-MS

采用电感耦合等离子体质谱(ICP-MS)对三七血伤宁散(SQXSNP)中无机元素组成进行分析并建立相关指纹图谱,为产品质量均一性和安全性评价提供参考。以HNO_(3)为消解溶剂,采用ICP-MS法定量测定三七血伤宁散中无机元素的含量,通过无机元素特征图谱,Pearson相关系数对结果进行分析;通过聚类分析结合主成分分析(PCA)对质量均一性进行评价。三七血伤宁散中无机元素以Mg、Fe、Al、Mn和Zn为主,且不同元素含量之间具有一定相关性。Cu和As这两种重金属元素的限量符合药典相关限度规定。不同批次的样品谱图均有相似的峰形。指纹图谱、聚类分析及主成分分析结果表明,三七血伤宁散中无机元素的质量均一性较好,主要特征元素为Li、Ga、Fe、Mg、V、Ni和Sr。本研究操作方法简便、分析速度快、准确度高,适用于三七血伤宁散中无机元素的测定,可为三七血伤宁散的质量评价和综合利用提供理论依据。

To analyze the inorganic elements composition in Sanqi Xueshangning powder(SQXSNP)by inductively coupled plasma-mass spectrometer(ICP-MS)method,establish the fingerprint of inorganic elements,and provide the research basis for the quality uniformity and safety evaluation.Nitric acid was used as the digestion reagent,the content of inorganic elements was determined by ICP-MS.The results were analyzed by Heatmaps and Pearson correlations.The qualityuniformity was evaluated by cluster analysis and principal components analysis(PCA)for different batches of SQXSNP.The main inorganic elements in SQXSNP included Mg,Fe,Al,Zn and Mn,and there was a certain correlation among different elements.The content of 2 heavy metal elements,including Cu,As,were below the limit of heavy metals and harmful elements.Different batches of samples had similar peak shape,and the content of inorganic elements content in different orders tended to be consistent.The results of fingerprint similarity,cluster analysis,and PCA showed that the inorganic elements showed good qual-ity uniformity,and Li,K,Fe,Mg,V,Ni,and Sr might be the characteristic elements in SQXSNP.The method is simple,rapid and accurate,and can be used for the determination of 16 trace elements in SQXSNP in this study.Which provides a theoretical basis for quality assessment and comprehensive utilization of SQXSNP.