口服麻杏石甘散对BRD犊牦牛血清指标的影响

Effects of Oral Administration Maxingshigan Powder on Serum Indexes of BRD Yak Calves

研究探讨了麻杏石甘散灌服对犊牦牛呼吸道疾病(bovine respiratory disease,BRD)的治疗效果及血清中氧化应激指标和炎性指标的影响,为其在临床应用提供理论依据。选择12头表现BRD症状的犊牦牛,以0.75 g/kg剂量温热水冲调灌服麻杏石甘散,每天1次,连用6 d,以5头健康犊牛正常饲喂作为空白对照,用药前(第0天),药后第3、6天记录犊牦牛临床症状并根据WI评分表进行打分评价,颈静脉采血测定血清中肿瘤坏死因子-α(TNF-α)、溶菌酶(LYZ)、白细胞介素-6(IL-6)、乳铁蛋白(LTF)、血清淀粉样蛋白A(SAA)等5种抗炎及抗氧化应激指标水平。结果表明,用药后第6天,WI评分较第3天显著性降低(P<0.05),治愈率为83.3%。通过6 d治疗,患病组犊牛血清中TNF-α、LZM、IL-6、LTF、SAA等抗炎及抗氧化指标浓度与健康组差异不显著(P>0.05)。可见,在本试验条件下,麻杏石甘散能有效改善犊牦牛BRD症状,缓解炎性反应及氧化应激症状,对治疗牦牛BRD具有一定的价值,可用于高寒地区牦牛及相关草食动物呼吸道疾病治疗。

This study investigated the therapeutic effects of Maxingshigan powder on bovine respiratory disease(BRD)and its effects on serum oxidative stress indexes and inflammatory indexes,so as to provide a theoretical basis for clinical application.12 yak calves with BRD symptoms were selected and given Maxingshigan powder at 0.75 g/kg dose in warm water once a day for 6 days,and 5 healthy calves were fed normally as the control.On the 0,3 and 6 days of medication,the clinical symptoms of yak calves were recorded and scored according to WI scoring table,and blood was collected from jugular vein.The serum levels of tumor necrosis factor-α(TNF-α),lysozyme(LYZ),interleukin-6(IL-6),lactoferrin(LTF)and serum amyloid A(SAA)were determined after centrifugation.Results showed that WI score on the 6th day after medication was significantly lower than that on the 3rd day(P<0.05),the cure rate was 83.3%on the 6th day after medication.After 6 days of treatment,the serum concentrations about anti-inflammatory and antioxidant indexes of TNF-α,LZM,IL-6,LTF,SAA in sick group were not significantly different from those in healthy group(P>0.05).In conclusion,Maxingshigan powder can effectively improve the BRD symptoms of yak calves,relieve the inflammatory response and oxidative stress symptoms,and has certain reference value for the treatment of BRD in yaks.This method is suitable for the treatment of respiratory diseases in yaks and related herbivores in alpine area.