球参酶解物馏分对秀丽隐杆线虫抗衰老和抗氧化的作用

Anti-aging and Antioxidant Activities of Hydrolysate Fraction from Spherical Sea Cucumber(Phyllophorus proteus)in Caenorhabditis elegans

为探究球参酶解物馏分(Phyllophorus proteus hydrolysate fraction,PPHF)对秀丽隐杆线虫(线虫)的抗衰老和抗氧化作用,分别利用0.5、2.0 mg/mL和8.0 mg/mL PPHF喂食线虫,考察线虫生理生化指标的变化规律。结果表明,制备的PPHF呈米黄色,其L*值和b*值分别81.28和15.88。PPHF中分子质量为180~1 000 Da的寡肽占比达到63.76%,谷氨酸和甘氨酸的占比分别为19.50%和15.34%。喂食0.5、2.0 mg/mL和8.0 mg/mL PPHF后,线虫的寿命在正常条件下分别延长22.43%、54.60%和78.89%,在热应激和氧化应激条件下也得到延长。喂食0.5 mg/mL PPHF可以显著提高线虫的身体长度,而喂食2.0 mg/mL PPHF才能显著提高线虫的吞咽频率。喂食0.5 mg/mL和8.0 mg/mL PPHF的线虫身体弯曲频率在培养第2天时比空白组分别提高了7.75%和43.71%。PPHF能够显著降低线虫体内活性氧含量并提高抗氧化酶活力、还原型谷胱甘肽含量和总抗氧化能力,且呈剂量依赖性。综上,PPHF可以通过提高线虫体内抗氧化能力,进而改善生理功能并延长寿命。

To explore anti-aging and antioxidant activities of Phyllophorus proteus hydrolysate fraction(PPHF)on Caenorhabditis elegans,the physiological and biochemical indicators of C.elegans fed 0.5,2.0 mg/mL and 8.0 mg/mL PPHF were determined.the results showed that ppHF was beige in color,and its L*and b*values were 81.28 and 15.88,respectively.The proportion of oligopeptides with molecular mass of 180–1000 Da in PPHF was 63.76%,and the proportions of glutamic acid and glycine were 19.50% and 15.34%,respectively.After being fed 0.5,2.0,and 8.0 mg/mL PPHF,the lifespan of C.elegans was increased by 22.43%,54.60%,and 78.89%,respectively,under normal conditions,and also increased under thermal or oxidative stress.the body length of C.elegans was increased significantly by feeding 0.5 mg/mL ppHF,while the swallowing frequency was significantly increased by feeding 2.0 mg/mL PPHF.the body bending frequency of C.elegans fed 0.5 and 8.0 mg/mL PPHF was increased by 7.75%and 43.71%,respectively,when compared with the control group on the second day of incubation.PPHF significantly reduced the content of reactive oxygen species(ROS)in C.elegans,and increased the antioxidant enzyme activity,glutathione content and total antioxidant capacity in a dose-dependent manner.these results indicated that PPHF could improve the physiological function and prolong the lifespan by enhancing antioxidant capacity in C.elegans.