微流控芯片制备载镁水凝胶核-壳微球的体外成骨活性研究

Osteogenic activity of magnesium loaded hydrogel core-shell microspheres by microfluidic chip in vitro

目的:探索载镁水凝胶微球的在体外细胞实验中的成骨活性。方法:设计和搭载微流控芯片装置,并利用该装置载镁水凝胶核-壳微球精准调控镁离子在体外稳定释放,系统研究可控镁离子释放促进成骨活性机制。结果:微流控芯片制备出的载镁水凝胶微球(PLGA/MgO-alginate微球)呈现单分散性和特殊的核-壳结构调控镁离子在体外稳定释放的浓度稳定在50 ppm。不仅能显著增强MC3T3-E1前成骨细胞的活性和增殖能力,还可以改善其成骨分化和矿化能力,并增强了ALP活性。结论:载镁水凝胶核-壳微球能显著提高MC3T3-E1前成骨细胞的成骨活性。

Objective:To explore the osteogenic activity of in vitro cultured preosteoblasts treated by magnesium loaded hydrogel core-shell microspheres.Methods:A microfluidics chip device was designed and made to fabricate magnesium loaded hydrogel core-shell microspheres to precisely control the stable release of magnesium ions in vitro,the mechanism of controllable magnesium ion release in the promotion of osteogenic activity of MC3T3-E1 preosteoblasts was systematically investigated.Results:The magnesium loaded hydrogel microspheres(PLGA/MgO-alginate microspheres)prepared by the microfluidics chip exhibited to be monodisperse with special core-shell structure and could control the stable release of magnesium ions at a concentration of 50 ppm in vitro.The in vitro study showed that it significantly enhanced the activity and proliferation ability of MC3T3-E1 preosteoblasts,promoted their osteogenic differentiation and mineralization ability,and increased ALP activity.Conclusion:Magnesium loaded hydrogel core-shell microspheres can promote the osteogenic activity of MC3T3-E1 osteoblasts in vitro.