摘要
利用HCl和十六烷基三甲基溴化铵(CTAB)对凹凸棒土进行改性,增加其表面功能基团,研究改性材料对脱氧核糖核酸(DNA)的吸附与解吸效果。通过X射线衍射(XRD)仪、傅里叶变换红外光谱(FTIR)仪、扫描电子显微镜(SEM)和比表面积测试仪(BET)等对改性前后的凹凸棒土进行表征。以金黄色葡萄球菌为例,考察改性凹凸棒土用于核酸提取的得量、纯度及完整性。结果表明,改性材料对DNA的饱和吸附量可达18.657μg/mg,对金黄色葡萄球菌核酸提取得量为(5.45±0.46)ng/105 CFU,且结构完整、纯度高。
Attapulgite was modified with HCl and CTAB to increase functional groups on the surface of attapulgite,and the adsorption and desorption of modified materials on DNA were studied.X-ray diffractometer(XRD),Fourier transform infrared spectroscopy(FTIR)and scanning electron microscope(SEM)were used to characterize attapulgite before and after modification.The amount,purity and integrity of nucleic acid extraction were investigated with Staphylococcus aureus as the object of nucleic acid extraction.The results showed that the saturated adsorption capacity of the modified material for DNA could reach 18.657μg/mg,the nucleic acid extraction capacity of Staphylococcus aureus was(5.45±0.46)ng/105 CFU,the structure was intact and the purity was high.
作者
臧垚
王为彦
王莫凡
潘家昊
张孝杰
郭亚辉
Zang Yao;Wang Weiyan;Wang Mofan;Pan Jiahao;Zhang Xiaojie;Guo Yahui(Xuyi Attapulgite Application Technology Research and Development and Achievement Transformation Center,Xuyi,Jiangsu 211700;Schoolof Food Science and Technology,Jiangnan University,Wuxi,Jiangsu 214122;Xuyi Attapulgite R&D Center,Huaiyin Institute of Technology,Huaian,Jiangsu 211700)
出处
《非金属矿》
2024年第3期63-66,71,共5页
Non-Metallic Mines
基金
淮安市科技计划项目(HAB202378)
江南大学大学生创新创业训练计划项目(校重点项目)。
关键词
凹凸棒土
核酸提取
吸附
改性
attapulgite
DNA extraction
adsorption
modification
