水稻矮缩病毒对黑尾叶蝉取食及产卵偏好性的影响

Effects of rice dwarf virus on feeding and oviposition preference of Nephotettix cincticeps(Uhler)(Hemiptera∶Cicadellidae)

水稻矮缩病毒(Rice dwarf virus,RDV)是水稻普通矮缩病(Rice dwarf disease)的病原,主要依赖黑尾叶蝉Nephotettix cincticeps经卵以持久增殖方式传播。本研究采用室内试验观测了带毒与无毒的黑尾叶蝉在健康及感病水稻间的取食、产卵选择趋性。取食偏好性试验发现,无毒叶蝉对感病水稻趋性更强,从观测的第8小时开始达显著差异水平;带毒叶蝉则对健康水稻趋性更强,在观测的第24小时开始达到显著差异水平,表明RDV侵染水稻会引起黑尾叶蝉取食偏好性的改变。产卵偏好性试验发现无毒及带毒黑尾叶蝉在健康及感病水稻间3个观测指标(每植株卵块数、每卵块的卵量及每植株上的总卵量)均无显著差异,表明RDV侵染水稻对黑尾叶蝉产卵选择偏好性无显著影响。本文初步明确RDV对黑尾叶蝉取食和产卵偏好性的影响,为揭示介体昆虫-病毒-寄主植物三者之间的互作机制提供佐证。

Rice dwarf virus(RDV)is the pathogeny of rice dwarf virus disease.It is mainly transmitted by green rice leafhopper(GRLH),Nephotettix cincticeps(Uhler)(Hemiptera∶Cicadellidae),in a persistent-propagative manner and is transovarially transmitted to offspring.Host plant selection preference for feeding and oviposition of non-viruliferous or viruliferous(carrying RDV)GRLHs between RDV-free and RDV-infected plants were tested through laboratory experiment.In the host plant feeding selection bioassay,more non-viruliferous GRLHs were attracted to RDV-infected rice plants,reaching a significant difference level from the 8 h after inoculation.More viruliferous GRLHs preferred to located on RDV-free rice plant for feeding,with the differences at significant level at 24 h after inoculation.These results indicated that RDV could altering their vector s host plant feeding selection preference.In the host oviposition preference bioassay,there were no significant differences in the three parameters(No.of egg mass/plant,egg number/egg mass and total No.of egg/plant)between RDV-free and RDV-infected rice plants,which indicated that RDV posing a negligible risk to GRLH oviposition preference.In this study,the effects of RDV on GRLHs host plant selection for feeding and oviposition were preliminarily clarified.We expect to providing evidence for reveal the mechanism within the tritrophic relationship.