HAUS5在肾透明细胞癌中的表达及其对预后的意义

Expression and prognostic significance of HAUS5 in renal clear cell carcinoma

目的探讨肾透明细胞癌中奥格明类复合亚基5(HAUS5)基因的表达及其对预后的意义。通过生物信息学方法和多种分析手段,揭示其在肾透明细胞癌发生、发展中的潜在机制。方法通过TIMER2网站进行泛癌分析,利用人蛋白质图谱数据库获取基于免疫组织化学的肾透明细胞癌抗体特异性染色评分。同时,从肿瘤基因图谱数据库下载关于肾透明细胞癌的基因mRNA表达量及相关临床数据。通过R语言及Excel软件整理得到HAUS5基因在肾透明细胞癌中的mRNA表达量及相关临床数据。根据中位HAUS5表达量将患者分为高表达组和低表达组。使用SPSS27.0和R语言等软件进行生存分析和功能分析,并进行可视化。结果HAUS5在肾透明细胞癌组织和细胞中均呈高表达,肾透明细胞癌HAUS5表达越高发生死亡的风险越高,差异有统计学意义(风险比=1.744,95%可信区间:1.286~2.365,P<0.001)。对整体生存明显相关的其他临床变量[远处转移(M分期)、临床分期(stage分期)、肿瘤原发灶状况(T分期)]也有影响(风险比=1.551、1.866、1.891,95%可信区间:1.302~1.846、1.637~2.126、1.608~2.225,P<0.001)。结论HAUS5在肾透明细胞癌组织和细胞中均呈高表达,且与肾透明细胞癌的进展及不良预后相关,是临床治疗肾透明细胞癌的潜在靶点。

Objective To investigate the expression of Augmin like complex subunit 5(HAUS5)gene and its prognostic significance in renal clear cell carcinoma(RCCC),elucidating its potential mechanisms in the occurrence and development of RCCC through bioinformatics methods and various analytical approaches.Methods Pan-cancer analysis was conducted using the TIMER2 website.Human protein atlas database was utilized to obtain immunohistochemistry-based antibody-specific staining scores for RCCC.Additionally,gene mRNA expression levels and related clinical data for RCCC were downloaded from the Cancer Genome Atlas database.mRNA expression levels and associated clinical data of HAUS5 gene in RCCC were organized using R and Excel software.Patients were categorized into the high and the low expression groups based on the median HAUS5 expression level.SPSS27.0 and R software were used for survival analysis,functional analysis,and visualization.Results HAUS5 exhibited high expression in both RCCC tissues and cells.Higher expression of HAUS5 in RCCC was associated with an increased risk of mortality,with statistically significant differences(hazard ratio=1.744,95%confidence interval:1.286-2.365,P<0.001).Other clinical variables significantly associated with overall survival[such as distant metastasis(M stage),clinical stage,and primary tumor status(T stage)]also had an impact(hazard ratio=1.550,1.866,1.891,95%confidence interval:1.302-1.846,1.637-2.126,1.608-2.225,P<0.001).Conclusion HAUS5 is highly expressed in both RCCC tissues and cells and is closely associated with the progression and adverse prognosis of RCCC,suggesting it as a potential target for clinical treatment of RCCC.