长链非编码RNA-EPIC1与前列腺癌淋巴结转移及预后关系

The relationship between long non-coding RNA-EPIC1 with lymph node metastasis and prognosis in prostate cancer

目的探讨长链非编码RNA-EPIC1与前列腺癌(PC)淋巴结转移及患者预后关系。方法用RT-PCR分别检测EPIC1在正常前列腺上皮细胞系(RWPE-1)和4种PC细胞系表达差异,以及9例PC组织及癌旁组织中EPIC1表达差异,分析EPIC1与83例PC患者临床病理和生存资料之间关系。结果EPIC1在PC细胞系中的表达水平明显高于RWPE-1(P<0.05),在患者PC组织表达水平也高于癌旁组织(P<0.05)。EPIC1表达程度与肿瘤Gleason评分、TNM分期和淋巴结转移明显相关(P<0.05),但与患者年龄、PSA水平无相关性(P>0.05)。生存分析显示EPIC1高表达和低表达患者5年生存率分别为37%、59%(P=0.017)。结论EPIC1的表达在PC中异常增高,EPIC1高表达患者预后较差,EPIC1可能作为PC肿瘤转移和判断预后的有效辅助指标。

Objective To explore the relationship between novel long non-coding RNA-EPIC1 and lymph node metastasis and prognosis of prostate cancer(PC).Methods The expression of EPIC1 in normal prostatic epithelial cell lines and four PC cell lines were detected by RT-PCR,and the level of EPIC1 in nine PC tissues and paired adjacent normal tissues were also detected.The relationship between EPIC1 with clinical pathology and survival data of 83 PC patients were also analyzed.Results The level of EPIC1 in PC cell line was significantly higher than that in normal prostate epithelial cell line,and the expression level in PC tissue was also higher than that in adjacent normal tissue(P<0.05).The expression of EPIC1 was significantly correlated with Gleason score(P<0.05),TNM stage and lymph node metastasis(P<0.05),but not with age and PSA level(P>0.05).Survival analysis showed that the 5-year survival rates of patients with high and low expression of EPIC1 were 37%and 59%respectively,there was significant difference between the two groups(P=0.017).Conclusion The expression of EPIC1 is abnormally increased in PC,and the prognosis of patients with high level of EPIC1 is poor.EPIC1 may be used as an effective adjuvant marker for metastasis and prognosis of PC.