后处理加工工艺对饲用凝结芽孢杆菌活菌收率的影响

Effects of the Post-processing Technique on Recovery Rate of Viable Bacteria of Bacillus coagulans for Feeding

【目的】探明提高饲用凝结芽孢杆菌发酵后处理加工贮存过程中的活菌收率,为降低规模化生产成本提供科学依据。【方法】以饲用凝结芽孢杆菌(Bacilluscoagulans)W-02菌株为研究对象,分别检测凝结芽孢杆菌发酵后处理加工贮存过程中离心浓缩工艺、干燥方式、粉碎时间和低温保存时间前后活菌数,计算各环节活菌收率,最终计算产品活菌总收率。【结果】经4000 r/min、15 min离心浓缩工艺,凝结芽孢杆菌菌泥平均活菌收率为91.57%;经-58℃、100 mT真空冷冻干燥工艺,干燥产品活菌收率为92.98%;经转速24000 r/min、粉碎15 s,粉碎产品活菌收率为83.37%;经4℃低温保存365 d,凝结芽孢杆菌活菌收率为71.21%。【结论】饲用凝结芽孢杆菌发酵液经离心浓缩、吸附干燥、高速粉碎最优条件处理后,低温保存365 d时活菌总收率(各环节活菌收率之积)为50.55%。

【Objective】It aimed to study the development of recovery rate of viable bacteria of Bacillus coagulans for feeding during processing and storage after fermentation,which provided the scientific basis for reducing the cost of large-scale production.【Method】Taking the B.coagulans for feeding,W-02,as the study object,the centrifugal concentration process,drying method,smashing time and the viable bacterial before and after cryopreservation time during processing and storage after fermentation,and the total recovery rates of viable bacteria were counted.【Result】The average recovery rate of viable bacteria of B.coagulans was 91.57%after centrifugal concentration by 4000 r/min for 15 min.The recovery rate of viable bacteria in dry B.coagulans was 92.98%by vacuum freeze-drying at-58℃and 100 mT.The recovery rate of viable bacteria in crushed B.coagulans was 83.37%through the smashing process at 24000 r/min and 15 seconds.The recovery rate of viable bacteria of B.coagulans was 71.21%as stored at 4℃for 365 d.【Conclusion】After centrifugal concentration,sorption drying,high-speed crushing,the total recovery rate of viable bacteria of B.coagulans for feeding(the product of viable bacteria at each stage)is 50.55%as stored for 365 d with low temperature.