草珊瑚与海南草珊瑚叶绿体基因组结构及系统发育关系分析

Analysis of the Genome Structure and Phylogenetic Relationship Between Sarcandra glabra and Sarcandra glabra subsp.brachystachys

利用Illumina全基因组测序数据组装了草珊瑚和海南草珊瑚叶绿体全基因组,两者总GC含量均为39%;草珊瑚叶绿体基因组总大小为158881 bp,包含一个88169 bp的大单拷贝区(LSC)、一个18446 bp的小单拷贝区(SSC)和一对26133 bp的反向重复区(IRs);海南草珊瑚叶绿体基因组总大小为158826 bp,包含一个88104 bp的LSC、一个18440 bp的SSC和一对26141 bp的IRs;二者均注释得到128个基因,包括84个蛋白编码基因(CDS)、8个rRNA基因和36个tRNA基因;系统发育树表明,草珊瑚叶绿体基因组与NCBI下载的两个草珊瑚基因组序列聚为一支,再与海南草珊瑚聚为一支,草珊瑚属与金粟兰属互为姐妹群。

The whole chloroplast genomes of Sarcandra glabra and Sarcandra glabra subsp.brachystachys were assembled using Illumina whole genome sequencing data,and the total GC content of both was 39%.The total chloroplast genome size of Sarcandra glabra was 158881 bp,including a large single copy region(LSC)of 88169 bp,a small single copy region(SSC)of 18446 bp and a pair of reverse repeat regions(IRs)of 26133 bp.The total chloroplast genome size of Sarcandra glabra subsp.brachystachys was 158826 bp,including 88104 bp LSC,18440 bp SSC and 26141 bp IRs.128 genes,including 84 CDS,8 rRNA genes and 36 tRNA genes,were annotated by both plants.Phylogenetic tree showed that chloroplast genome of Sarcandra glabra was clustered with two Sarcandra glabra genome sequences downloaded by NCBI,and the support rate was 100%,and then the Sarcandra glabra subsp.brachystachys together into a sister group,and the Sarcandra and Chloranthus were sister groups each other.