中药混合液对耐甲氧西林金黄色葡萄球菌的体外抗菌作用的研究

Study on Antibacterial Effect of Chinese Medicine Mixture on Methicillin-Resistant Staphylococcus Aureus in Vitro

目的探究中药混合液对耐甲氧西林金黄色葡萄球菌(Methicillin-resistant Staphylococcus Sureus,MRSA)的体外抗菌作用。方法选取2022年1月—2023年6月江苏省启东市中医院检验科临床分离的40株MRSA。万古霉素、中药混合液对MRSA的体外最低抑菌浓度(Minimum Inhibitory Concentration,MIC)值采用微量肉汤稀释法测定;用K-B纸片扩散法测定万古霉素和中药混合液对MRSA的抑菌效果,探究不同药物浓度对MRSA抑菌活性的影响。结果万古霉素MIC值为(1.56±0.55)mg/L,低于中药混合液的(1.63±0.62)mg/mL,差异有统计学意义(P<0.05)。万古霉素抑菌圈直径为(20.56±0.87)mm,优于中药混合液的(19.89±0.73)mm,差异有统计学意义(P<0.05)。抑菌圈直径大小随浓度增加而增大且中药混合液各浓度抑菌圈直径均大于万古霉素,差异有统计学意义(P均<0.05)。结论中药混合液对MRSA的体外最低抑菌浓度极低,可以有效抑制耐甲氧西林金黄色葡萄球菌的生长。

Objective To explore the antibacterial effect of Chinese herbal mixture on methicillin-resistant Staphylococcus aureus(MRSA)in vitro.Methods 40 strains of MRSA clinically isolated from the clinical Laboratory Department of Qidong Hospital of Traditional Chinese Medicine,Jiangsu Province from January 2022 to June 2023 were selected.The value of minimum inhibitory concentration(MIC)of vancomycin and Chinese medicine mixture on MRSA in vitro was determined by microbroth dilution method.K-B disk diffusion method was used to determine the antibacterial effect of vancomycin and Chinese medicine mixture on MRSA,and to explore the influence of different drug concentrations on the antibacterial activity of MRSA.Results The MIC value of vancomycin was(1.56±0.55)mg/L,lower than that of traditional Chinese medicine mixture(1.63±0.62)mg/mL,and the difference was statistically significant(P<0.05).The diameter of the inhibition zone of vancomycin was(20.56±0.87)mm,better than that of the traditional Chinese medicine mixture(19.89±0.73)mm,and the difference was statistically significant(P<0.05).The diameter of the inhibition zone increased with the increase of the concentration,and the diameter of the inhibition zone of each concentration of the traditional Chinese medicine mixture was greater than that of vancomycin,and the differences were statistically significant(all P<0.05).Conclusion The minimum inhibitory concentration of Chinese medicine mixture on MRSA in vitro is very low,which can effectively inhibit the growth of methicillin-resistant Staphylococcus aureus.