浒苔多糖对高脂饮食小鼠的保护作用研究

Study on The Protective Effect of Enteromorpha Prolifera Polysaccharides on High-Fat Diet Mice

目的:通过建立高脂饮食小鼠模型,研究浒苔多糖对高脂血症小鼠的保护作用。方法:取健康成年昆明种雄鼠60只,随机分为正常组、模型组、辛伐他汀组、浒苔多糖高、中、低剂量组。正常组给予普通饲料,其余各组给予高脂饲料饲养42d。采用灌胃给药,浒苔多糖组予以300、200、100mg/kg剂量,辛伐他汀组予以3mg/kg剂量,正常组和模型组予以同等体积生理盐水,每日1次。试验结束后,检测血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、总蛋白(TP)、白蛋白(ALB)、总胆红素(T-bil-V)、直接胆红素(D-bil-V)。结果:浒苔多糖能显著降低高脂饮食小鼠体质量和肝脏指数,降低血清中TC、TG、LDL-C、ALT、AST、T-bil-V和D-bil-V水平,提高HDL-C水平。结论:浒苔多糖能降低高脂饮食小鼠血清中的血脂水平,改善肝功能。

【Objective】To study the protective effect of Enteromorpha prolifera polysaccharides on hyperlipidemia mice by establish⁃ing a high-fat diet mouse model.【Method】Totally 60 healthy adult Swiss male mice were randomly divided into normal group,model group,Simvastatin group,Enteromorpha prolifera polysaccharide high,medium and low dose groups.The normal group was given regular beverages,while the other groups were fed with high-fat feed for 42 days.Enteromorpha prolifera polysaccharide group was given 300mg/kg,200mg/kg,100mg/kg by gavage,Simvastatin group was given 3mg/kg,normal group and model group were given the same volume of normal saline once a day.After the test,total cholesterol(TC),triglyceride(TG),low-density lipoprotein(LDL-C),High-density lipoprotein(HDL-C),Alanine transaminase(ALT),aspartate Transaminase(AST),Alkaline phosphatase(ALP),total protein(TP),albumin(ALB),total bilirubin(T-bil-V),and direct bilirubin(D-bil-V)in the serum were detected.【Result】Enteromorpha prolifera polysaccharides could significantly reduce body mass and liver index in high-fat diet mice,reduce serum levels of TC,TG,LDL-C,ALT,AST,T-bil-V,and increase HDL-C levels.【Conclusion】Enteromorpha polysaccharides can significantly reduce the blood lipid levels in the serum of mice on a high-fat diet and improve liver function.