草石蚕多糖的制备及其对高糖诱导大鼠肾小球系膜细胞的影响

Preparation of Polysaccharides from Stachys Sieboldii and their Effect on High Glucose-induced Rat Glomerular Mesangial Cells

为研究草石蚕多糖对高糖诱导大鼠肾小球系膜细胞(HBZY-1)的影响。草石蚕水提液经脱蛋白、AB-8大孔树脂柱层析分离,得草石蚕粗多糖并进行分级醇沉分离得到30%醇沉组分(SSMP-30%)、60%醇沉组分(SSMP-60%)和90%醇沉组分(SSMP-90%),其中SSMP-90%的含量最高。以高糖诱导HBZY-1为细胞模型对醇沉组分进行体外活性测试。结果表明,不同醇沉组分对模型细胞均无毒性,与高糖对照组比较,SSMP-30%和SSMP-90%组分对高糖诱导HBZY-1的增殖具有显著抑制作用(P<0.01),且呈浓度依赖性,其中SSMP-90%的活性最强。提示其对高糖诱导大鼠肾小球系膜细胞具有保护作用。

In order to study the effect of the polysaccharides from S.sieboldii on high glucose-induced glomerular mesangial cells(HBZY-1)in rats.The aqueous extract of S.sieboldii was deproteinised and separated by AB-8 macroporous resin column chromatography to obtain the crude polysaccharides of S.sieboldii,and then subjected to graded alcoholic precipitation to obtain the 30%alcoholic precipitation fraction(SSMP-30%),60%alcoholic precipitation fraction(SSMP-60%)and 90%alcoholic precipitation fraction(SSMP-90%),in which SSMP-90%had the highest content.The in vitro activity of the alcohol precipitated fractions was tested using high glucose-induced HBZY-1 as a cell model.The results showed that the different alcohol precipitation fractions were non-toxic to the model cells,and compared with the high glucose control group,the SSMP-30%and SSMP-90%fractions had a significant inhibitory effect on the proliferation of high glucose-induced HBZY-1(P<0.01)in a concentration-dependent manner,in which SSMP-90%had the strongest activity.It was suggested that polysaccharides from S.sieboldii had a protective effect on high glucose-induced glomerular mesangial cells in rats.