柑橘黄酮对小鼠的降血糖作用研究

Study on the Hypoglycemic Effect of Citrus Flavonoids on Mice

目的:探究柑橘黄酮对四氧嘧啶诱导的糖尿病小鼠的降血糖作用。方法:原料为柑橘皮,将黄酮提取率作为本次研究指标,优化提取方法和工艺条件,通过四氧嘧啶腹腔注射的形式建立小鼠糖尿病模型,将其随机分组,即糖尿病模型组,格列本脲阳性组,不同剂量中高低的柑橘皮黄酮组,根据相应剂量来进行灌胃,对小鼠体重和血糖变化进行记录。结果:模型组没有通过治疗干预,其血糖值明显上升(P<0.05),连续10天灌胃后,高、中剂量组以及药物组血糖值明显比模型组更低,且中剂量组的整体降糖效果比高剂量组更显著。结论:柑橘黄酮能够显著降低糖尿病小鼠的血糖水平,为糖尿病的治疗和药物研发提供一定的理论基础。

Objective:To investigate the hypoglycemic effect of citrus flavonoids on diabetic mice induced by alloxouracil.Methods:The study utilized citrus peel as the raw material,with the extraction rate of flavonoids serving as the index.The extraction method and process conditions were optimized,followed by the establishment of a mouse diabetes model through intraperitoneal injection of alloxan.Subsequently,the mice were randomly divided into different groups:diabetes model group,glibenuride positive group,and different dose citrus peel flavonoid group.Gavage was administered according to the corresponding dose while recording changes in body weight and blood sugar levels on mice.Results:The blood glucose levels in the model group exhibited a significant increase without any treatment intervention(P<0.05).Following 10 consecutive days of intragastric administration,the high-dose,medium-dose,and drug groups demonstrated significantly lower blood glucose levels compared to the model group.Moreover,the medium-dose group exhibited a more pronounced hypoglycemic effect than the high-dose group.Conclusion:citrus flavonoids effectively reduces blood sugar levels in diabetic mice,providing a theoretical foundation for diabetes treatment and drug development.