食品中菌落总数纸片法的方法验证及结果分析

Method Validation and Result Analysis of the Paper Method for Total Bacterial Colony in Food

目的:探究三种厂家的测试片的纸片法在食品菌落总数测定中的一致性,分析比较不同厂家测试片的实际应用效果,为食品检测机构提供参考依据。方法:采用GB 4789.2-2022《食品安全国家标准食品微生物学检验菌落总数测定》对三种厂家的菌落总数测试片,通过纸片法和倾注法对标准菌株菌悬液、人工污染样品进行菌落总数检测,运用统计学分析确定两种方法之间检测结果的显著性和相关性。结果 经统计学分析,两种方法在标准菌株菌悬液和人工污染样品检测结果之间无显著性差异(P>0.05),两种方法的检测结果相关性非常好(R2>0.996)。结论:三种厂家的菌落总数测试片法与倾注法的检测结果一致,菌落总数测试片更加快速、简便,减少检测流程,提高检测效率。不同厂家的测试片存在一定差异,实验室可根据样品特性选择合适的测试片。

Objective:This thesis aims at exploring the consistency of the paper method of three manufacturers'test pieces in the determination of total bacterial colony in food,carrying out the analysis and contrast of the actual application effects of different manufacturers'test pieces,and providing reference basis for food testing institutions.Method:GB 4789.2-2022 of National Food Safety Standard for Microbiological Examination of Food-Determination of Total Bacterial Colony is used to test the total bacterial colony of three manufacturers'bacterial colony test pieces.The detection of the total number of bacterial colonies in standard bacterial suspensions and artificially contaminated samples is conducted by using the paper method and pouring method,and the statistical analysis is adopted to determine the significance and correlation of the detection results between the two methods.Results:In accordance with the statistical analysis,no significant difference(P>0.05)can be found between the two methods in the detection results of standard bacterial suspension and artificially contaminated samples.The good correlation between the detection results of the two methods is shown(R2>0.996).Conclusion:The results of the total bacterial colony test piece method and the pouring method from the three manufacturers are consistent.The test piece for total bacterial colony is faster and simpler,which is able to reduce the detection process and improve the detection efficiency.Some differences are found to exist between test pieces from different manufacturers,and the laboratory can select appropriate test pieces in line with the sample characteristics.