三桠苦叶绿体基因组结构和序列特征分析

Analysis on structure and sequence feature of chloroplast genome from Melicope pteleifolia

为了探明岭南地区特色中药三桠苦的叶绿体基因组结构和序列特征,以三桠苦叶片为材料,采用试剂盒法提取DNA和构建测序文库,利用Novaseq平台进行高通量测序,再结合生物信息学手段进行序列拼接、注释和特征分析。结果显示:三桠苦叶绿体基因组为158992 bp的环状四分体分子,含有134个基因;在三桠苦叶绿体基因组内找到86个简单重复序列,主要是A/T单核苷酸重复;检测到26907个密码子,以A/T结尾的密码子有着更高的使用频次;序列比较分析表明,三桠苦与同科植物在非编码区存在更明显的序列变异;系统进化树揭示不同来源的三桠苦亲缘关系最近,但在基因组成和碱基序列上呈现出多样性。

To illustrate the structure and sequence characteristics of chloroplast genome from Melicope pteleifolia,the leaf of M.pteleifolia was used for DNA isolation and library construction.The high-throughput sequencing was fulfilled by Novaseq platform,and then acquired sequence was assembled,annotated and analyzed using bioinformatics means.The results revealed that the chloroplast genome of M.pteleifolia was a circular quadripartite molecule with a size of 158992 bp and encoded 134 genes.Altogether 86 simple sequence repeat was found in the plastome and the A/T mononucleotide was predominate type.Total 26907 codon was screened out and those ended with A/T were frequently used.Sequence comparison demonstrated more divergence and occurred in non-coding region between M.pteleifolia and other Rutaceae species.Phylogenetic tree indicated various chloroplast genome sequences of M.pteleifolia shared close relationship and exhibited diversity in gene composition and base sequence.