芫根多糖的提取及其抗缺氧能力研究

Study on Extraction and Anti-Hypoxia Ability of Polysaccharides from Brassica rapa L.

为充分利用芫根资源,采用水提醇沉的方法提取芫根多糖,根据单因素试验和正交试验研究并优化芫根多糖提取工艺,并初步探究芫根多糖对急性缺氧小鼠血清中生化指标的影响。研究结果表明,芫根多糖的最佳提取工艺为料液比1∶20、提取温度100℃、提取时间4 h和提取次数4次。对于低压低氧环境中的小鼠,在给予芫根多糖干预后,小鼠血清中的氧化应激指标水平有所改善,表明芫根多糖具有一定的抗高原缺氧的作用。该研究结果将有助于深入研究芫根多糖的生物活性,为其在医学和保健食品工业等领域的应用提供科学支持。

In order to make full use of Brassica rapa L.resources,polysaccharides are extracted from Brassica rapa L.by water extraction and alcohol precipitation method.The extraction process of polysaccharides from Brassica rapa L.is studied and optimized by single factor test and orthogonal test,and the effects of polysaccharides from Brassica rapa L.on biochemical indexes in the serum of mice with acute hypoxia are explored preliminarily.The results show that the optimal extraction process of polysaccharides from Brassica rapa L.is solid-liquid ratio of 1∶20,extraction temperature of 100℃,extraction time of 4 h and extraction times of 4.For mice in low-pressure and low-oxygen environment,the levels of oxidative stress indexes in the serum of mice are improved after the intervention of polysaccharides from Brassica rapa L.,which indicates that polysaccharides from Brassica rapa L.have certain anti-hypoxia effect at high altitude.The research results will be helpful to study the bioactivity of polysaccharides from Brassica rapa L.in depth,and provide scientific support for their application in the fields of medicine and health food industry.