银杏叶提取物对单侧输尿管梗阻大鼠肾间质纤维化的影响及机制

The effect and mechanism of ginkgo biloba extract on renal interstitial fibrosis in rats with unilateral ureteral obstruction

目的探究银杏叶提取物(GBE)对单侧输尿管梗阻(UUO)大鼠肾间质纤维化(RIF)的影响及机制。方法随机选取20只SD大鼠作为Sham组,54只SD大鼠UUO建模成功后随机均分为模型组、GBE组和厄贝沙坦组。观察各组大鼠肾组织病理变化,比较各组大鼠血肌酐(SCr)、尿素氮(BUN)、超氧化物歧化酶(SOD)、丙二醛(MDA)、活性氧(ROS)水平及肾组织纤维连接蛋白(FN)、α-平滑肌肌动蛋白(α-SMA)、I型胶原蛋白(Col-I)、肝细胞生长因子(HGF)、转化生长因子-β1(TGF-β1)的表达。结果与Sham组比较,其他组SCr、BUN、24 h尿蛋白、MDA、ROS水平及FN、α-SMA、Col-I、TGF-β1表达显著升高,SOD水平及HGF表达显著下降(P<0.05)。与模型组比较,GBE组和厄贝沙坦组SCr、BUN、24 h尿蛋白、MDA、ROS水平及FN、α-SMA、Col-I、TGF-β1表达显著下降,SOD水平及HGF表达显著升高(P<0.05)。GBE组SCr、BUN、24 h尿蛋白、MDA、ROS水平低于厄贝沙坦组,SOD高于厄贝沙坦组(P<0.05)。结论GBE可抑制UUO大鼠RIF发生及进展,保护肾功能,其机制可能与纠正HGF/TGF-β1平衡失调有关。

Aim To explore the effect of ginkgo biloba extract(GBE)on renal interstitial fibrosis(RIF)in rats with unilateral ureteral obstruction(UUO)and mechanisms.Methods 20 SD rats were randomly selected as Sham group.54 SD rats were treated with UUO and randomly divided into model group,GBE group and irbesartan group,with 18 rats in each group.The pathological changes of renal tissue were observed,and the levels of serum creatinine(SCr),urea nitrogen(BUN),superoxide dismutase(SOD),malondialdehyde(MDA),reactive oxygen species(ROS),and the expression of fibronectin(FN),α-smooth muscle actin(α-SMA),collagen I(Col-I)and hepatocyte growth factor(HGF)in renal tissue,were compared in each group.Results Compared with the Sham group,the levels of SCr,BUN,24 h urinary protein,MDA,ROS and the expressions of FN,α-SMA,Col-I and TGF-β1 were significantly increased in the other group,while the levels of SOD and HGF were significantly decreased(P<0.05).Compared with the model group,the levels of SCr,BUN,24 h urinary protein,MDA,ROS and the expressions of FN,α-SMA,Col-I and TGF-β1 in GBE group and irbesartan group were significantly decreased,while the levels of SOD and HGF were significantly increased(P<0.05).The levels of SCr,BUN,24 h urine protein,MDA,and ROS in the GBE group were lower than those in the irbesartan group,while SOD was higher in the irbesartan group(P<0.05).Conclusion GBE can inhibit the occurrence and progress of RIF and protect renal function in UUO rats,and its mechanism may be related to correcting the imbalance of hepatocyte growth factor(HGF)and transforming growth factor-1(TGF-β1).