RNF43 mRNA、VEGF在胃癌患者中的表达情况及其临床意义

Expression of RNF43 mRNA and VEGF in patients with gastric cancer and the clinical significance

目的探究环指蛋白43(RNF43)mRNA、血管内皮生长因子(VEGF)在胃癌患者中的表达情况及其临床意义。方法选取胃癌患者105例作为研究对象,检测其外周血RNF43 mRNA相对表达量以及胃癌组织VEGF蛋白的表达水平。比较不同临床病理特征患者RNF43 mRNA、VEGF蛋白表达水平,并采用Logistic回归分析、多重线性回归分析探讨VEGF和RNF43与胃癌患者临床病理指标的关系。结果不同脉管侵犯、淋巴结转移以及TNM分期患者的VEGF蛋白表达阳性占比差异存在显著性(P<0.05)。Logistic分析显示,脉管侵犯、淋巴结转移以及TNMⅣ期是胃癌患者VEGF蛋白表达阳性的独立危险因素(P<0.05)。存在脉管侵犯、淋巴结转移、未分化型、浆膜浸润以及TNMⅢ、Ⅳ期患者的RNF43 mRNA相对表达量偏低(P<0.05)。多重线性回归分析显示,脉管侵犯、淋巴结转移、浆膜浸润、未分化型以及TNMⅢ、Ⅳ期是RNF43 mRNA相对表达量降低的影响因素(P<0.05)。结论胃癌患者外周血RNF43 mRNA低表达和胃癌组织中VEGF蛋白阳性表达均与胃癌患者疾病进展有关,可协助临床胃癌诊断和疗效评估。

Aim To investigate the expression of RNF43 mRNA and vascular endothelial growth factor(VEGF)in patients with gastric cancer and its clinical significance.Methods 105 patients with gastric cancer were selected as research objects to detect the relative expression of RNF43 mRNA in peripheral blood and the expression level of VEGF protein in gastric cancer sections.The expression levels of RNF43 mRNA and VEGF protein in different clinicopathological patients were compared,and Logistic regression analysis and multiple linear regression analysis were used to explore the relationship between VEGF and RNF43 and clinicopathologic indicators in patients with gastric cancer.Results The VEGF protein expression levels were significantly different in patients with different vascular invasion,lymph node metastasis and TNM stage(P<0.05).Logistic analysis showed that vascular invasion,lymph node metastasis and TNM stageⅣwere independent risk factors for positive expression of VEGF protein in gastric cancer patients(P<0.05).There were lower RNF43 mRNA relative expression levels in patients with vascular invasion,lymph node metastasis,histological type,serous infiltration,and high TNM stage(P<0.05).Multiple linear regression analysis showed that vascular invasion,lymph node metastasis,serous infiltration,undifferentiated type and TNMⅢ,Ⅳstage were the factors affecting the relative expression level of RNF43 mRNA(P<0.05).Conclusion The low expression of RNF43 mRNA in peripheral blood of patients with gastric cancer and positive VEGF protein in gastric cancer tissues are related to the progression of the disease in patients with gastric cancer,which can be used to assist the clinical diagnosis and evaluation of therapeutic effect of gastric cancer.