超声造影定量参数联合外周血单个核细胞miR-34a、miR-146a对PTC诊断和淋巴结转移的评估价值

Evaluation value of quantitative parameters of contrast-enhanced ultrasound combined with peripheral blood mononuclear cell miR-34a and miR-146a in the diagnosis and lymph node metastasis of PTC

目的探讨超声造影定量参数联合外周血单个核细胞(PBMC)miR-34a、miR-146a对乳头状甲状腺癌(PTC)诊断和淋巴结转移的评估价值。方法选取104例PTC患者,根据病理诊断分为甲状腺癌组69例和良性组35例;根据是否发生淋巴结转移,将69例甲状腺癌患者分为未转移组33例和转移组36例。比较各组超声造影定量参数峰值强度(PI)、达峰时间(TTP)、平均通过时间(MTT)、曲线下面积(AUC)和PBMC中miR-34a、miR-146a水平。采用ROC分析超声造影参数联合P BMC miR-34a、miR-146a对PTC诊断和淋巴结转移的评估价值。结果与良性组比较,甲状腺癌组超声造影定量参数及P BMC miR-34a水平均降低(P<0.05),而miR-146a水平升高(P<0.05)。与未转移组比较,淋巴结转移组PI、AUC、miR-146a水平升高(P<0.05),而TTP、MTT、miR-34a水平降低(P<0.05)。超声造影定量参数与PBMC miR-34a、miR-146a对PTC诊断和淋巴结转移的AUC均高于各项指标单独检测值(P<0.05)。结论联合应用超声造影定量参数与PBMC miR-34a、miR-146a指标检测能增强PTC早期诊断与淋巴结转移的评估效能。

Aim To explore the diagnostic and lymph node metastasis evaluation value of contrast-enhanced ultrasound quantitative parameters combined with peripheral blood mononuclear cell(PBMC)miR-34a and miR-146a in papillary thyroid cancer(PTC).Methods 104 patients with PTC in our hospital were selected and divided into a thyroid cancer group of 69 cases and a benign group of 35 cases based on surgical pathological diagnosis.Based on whether or not lymph nodes have metastasized,69 thyroid cancer patients were divided into a non-metastatic group of 33 cases and a metastatic group of 36 cases.The peak intensity(PI),time to peak(TTP),mean transit time(MTT),area under curve(AUC),and levels of miR-34a and miR-146a in PBMC in different groups were compared.The evaluation value of contrast-enhanced ultrasound parameters combined with PBMC miR-34a and miR-146a in the diagnosis and lymph node metastasis of PTC was analyzed by using ROC curves.Results Compared with the benign group,the quantitative parameters and PBMC miR-34a levels in the thyroid cancer group were decreased(P<0.05),while miR-146a levels were increased(P<0.05).Compared with the non-metastatic group,the levels of PI,AUC,and miR-146a in the lymph node metastasis group were increased(P<0.05),while the levels of TTP,MTT,and miR-34a were decreased(P<0.05).The AUC of quantitative parameters of contrast-enhanced ultrasound and PBMC miR-34a and miR-146a for the diagnosis of thyroid cancer and lymph node metastasis was higher than the individual detection values of various indicators(P<0.05).Conclusion The combination of quantitative parameters of contrast-enhanced ultrasound and detection of miR-34a and miR-146a indicators in PBMC can enhance the early diagnosis and evaluation of lymph node metastasis in PTC.