IL-17F、ANKRD49在非小细胞肺癌中的表达及其临床意义

Expression and clinical significance of IL-17F and ANKRD49 in non-small cell lung cancer

目的分析白细胞介素-17F(IL-17F)和锚蛋白重复结构域49(ANKRD49)在非小细胞肺癌(NSCLC)组织中的表达及其临床意义。方法收集60例NSCLC患者NSCLC组织及癌旁正常组织。采取免疫组化法检测NSCLC组织、癌旁组织ANKRD49、IL-17F表达情况。采用多元线性回归分析ANKRD49、IL-17F表达与NSCLC临床病理特征的关系;绘制Kaplan-Meier生存曲线分析ANKRD49、IL-17F表达情况对患者生存率的影响。结果NSCLC组织ANKRD49、IL-17F表达阳性率高于癌旁组织(P<0.05)。TNMⅠ期、无淋巴结转移、高分化程度的NSCLC组织中IL-17F表达阳性率分别高于TNMⅡ和Ⅲ期、有淋巴结转移和低分化程度(P<0.05);TNMⅢ期、有淋巴结转移、低分化程度的NSCLC患者癌组织中ANKRD49表达阳性率分别高于TNMⅠ和Ⅱ期、无淋巴结转移和中高分化程度者(P<0.05)。TNM分期是NSCLC组织IL-17F表达的影响因素;淋巴转移、分化程度是NSCLC组织ANKRD49表达的影响因素(P<0.05)。NSCLC患者IL-17F表达阳性者生存率高于阴性者(P<0.05),而ANKRD49表达阳性者生存率低于阴性者(P<0.05)。结论IL-17F、ANKRD49在NSCLC患者癌组织中的表达较高,且均与NSCLC患者预后关系密切,可作为评估NSCLC患者预后的潜在靶点。

Aim To analyze the expression and clinical significance of interleukin-17F(IL-17F)and ankyrin repeat domain 49(ANKRD49)in non-small cell lung cancer(NSCLC)tissues.Methods NSCLC tissue and adjacent normal tissue from 60 NSCLC patients were collected.Immunohistochemical staining was used to detect the expression of ANKRD49 and IL-17F in NSCLC tissue and adjacent cancer tissues.The relationship between the expression of ANKRD49 and IL-17F and the clinical and pathological characteristics of NSCLC was analyzed by using multiple linear regression analysis.Kaplan-Meier survival curve analysis was performed to assess the impact of ANKRD49 and IL-17F expression on patient survival rates.Results The positive expression rates of ANKRD49 and IL-17F in NSCLC tissues were higher than those in adjacent tissues(P<0.05).The positive expression rates of IL-17F in NSCLC tissues with TNM stageⅠ,no lymph node metastasis,and high differentiation were higher than those in TNM stageⅡandⅢ,with lymph node metastasis,and low differentiation,respectively(P<0.05).The positive expression rates of ANKRD49 in NSCLC tissues with TNM stageⅢ,lymph node metastasis,and low differentiation were higher than those in TNM stageⅠandⅡ,no lymph node metastasis,and medium to high differentiation(P<0.05).TNM staging was a factor influencing the expression of IL-17F in NSCLC tissue.Lymphatic metastasis and differentiation degree were influencing factors of ANKRD49 expression in NSCLC tissue(P<0.05).The survival rate of NSCLC patients with positive IL-17F expression was higher than that of negative patients(P<0.05),while the survival rate of ANKRD49 positive patients was lower than that of negative patients(P<0.05).Conclusion IL-17F and ANKRD49 are highly expressed in NSCLC tissues and are closely related to the prognosis of NSCLC patients.They could to be used as potential targets for evaluating the prognosis of NSCLC patients.