山栀子不同部位活性成分含量及抗氧化活性

Content and Antioxidant Activity of Active Components in Different Parts of Gardenia jasminoides Ellis

为比较山栀子不同部位活性成分并评价各部位体外抗氧化活性,本文用超声辅助提取法提取山栀子不同部位有效成分,并测定得率;使用紫外-可见分光光度法测定总环烯醚萜、总藏红花素、总黄酮和总酚含量;利用高效液相色谱法测定山栀子不同部位活性成分单体;采用DPPH、ABTS+自由基清除试验评估其体外抗氧化能力,并进行活性成分与抗氧化活性的相关性分析。结果表明,山栀子叶中的总环烯醚萜和总酚含量最高,分别为244.87±5.89 mg·g^(-1)和16.71±0.55 mg·g^(-1);山栀子果中的总藏红花素(16.18±1.40 mg·g^(-1))相较于其余部分存在显著性差异(P<0.05),其中藏红花素-Ⅰ为藏红花苷主要形式,约占总藏红花素的60%;山栀子花中的总黄酮含量最高43.61±1.01 mg·g^(-1)。根据DPPH自由基、ABTS+自由基清除率试验,山栀子叶的抗氧化活性最好,IC50分别为36.00±2.70和31.96±3.87μg·mL-1;经相关性分析发现环烯醚萜类化合物与酚类化合物与抗氧化能力呈强相关性。采用超声辅助提取法提取得到的山栀子不同部位活性成分,发现不同部位均具有一定抗氧化能力,同时叶具有超越果的抗氧化活性,为天然抗氧化剂的开发提供了理论来源。

To compare the active compounds in different parts of Gardenia jasminoides Ellis and evaluate their in vitro antioxidant activities,this study used the ultrasound-assisted method to extract the active compounds in different parts of Gardenia jasminoides Ellis and measured the yield.Their total iridoids,total crocins,total flavonoids and total polyphenols were measured by UV-visible spectrophotometer method.Their active compound monomers were measured by HPLC method.DPPH and ABTS+radical scavenging methods were used to valuate their in vitro antioxidative activities then the correlation analysis was founded between the contents of active compounds and IC50 of DPPH and ABTS+radical.Gardenia jasminoides Ellis leaf had the highest total iridoids contents and total polyphenols contents,which were 244.87±5.89 mg·g^(-1) and 16.71±0.55 mg·g^(-1) respectively.Results showed that,theGardenia jasminoides Ellis fruit had the significantly highest total crocins contents(P<0.05),which was 16.18±1.40 mg·g^(-1),Crocin-I was the main form of crocin,which occupied about 60%of the total crocin contents.Gardenia jasminoides Ellis flower had the highest total flavonoids contents and,which was 43.61±1.01 mg·g^(-1).According to DPPH and ABTS+radical scavenging experiments,the Gardenia jasminoides Ellis leaf had the best antioxidative activities,which IC50 of DPPH and ABTS+were 36.00±2.70 and 31.96±3.87μg·mL-1.The correlation analysis showed that iridoids compounds and polyphenols compounds had strong correlation with antioxidative activities.Among the different parts of Gardenia jasminoides Ellis extracted by ultrasound-assisted method,it was found that different parts had certain antioxidant capacity.Gardenia jasminoides Ellis leaf had antioxidative activities beyond the fruit,which provided theoretical sources for development of natural antioxidants.